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Methods of isolating and selecting recombinant vaccinia viruses expressing heterogenetic viral antigens |
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| Authors: | R A Gibadulin V P Iuferov L F Lideman N A Parasiuk K S Ionova |
| Abstract: | The paper describes a method using plasmid construction pSC11 for generation of recombinant vaccinia viruses supporting coexpression of heterologous genes and beta-galactosidase. The Ca2+-phosphate method of cell transfection by recombinant DNAs generated on the basis of pSC11, and selection of recombinant viruses from blue plaques of virus-infected cells in the presence of X-gala are reported at length. |
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