肾综合征出血热患者循环内皮细胞CD95表达的临床研究

目的 探讨肾综合征出血热(HFRS)发热期和多尿期患者循环内皮细胞(CEC)中CD141、CD31及CD95的动态表达及临床意义.方法 应用流式细胞术分析不同型HFRS发热期和多尿期患者外周血细胞CD141、CD31及CD95的表达.多组数据的比较采用单因素方差分析.结果HFRS患者发热期和多尿期CD141+CD31+细胞(CEC)占外周血细胞的9.47%±1.98%和8.26%±1.55%.高于健康对照者的7.05%±1.45%(F=8.42;P=0.000,P=0.029),且发热期CD141+CD31+细胞较多尿期增高(P=0.48).HFRS发热期及多尿期患者CEC中CD95+细胞的平均荧光强度(MFI)较健康对照者明显升高(F=19.93;P=0.000,P=0.000),且发热期较多尿期明显增高(P=0.049).发热期各型患者CEC中CD95+细胞的MFI较健康对照者均明显增高(F=7.36;P=0.000),其中以重(危重)型患者最高,与中型和轻型比较差异有统计学意义(P=0.009,P=0.002).结论 CEC所占比例及CD95表达可能与HFRS病期及病情轻重有关.

Expression of CD95 on circulating endothelial cells in patients with hemorrhagic fever with renal syndrome

Objective To investigate the dynamic expressions and clinical significance of CD141, CD31 and CD95 on circulating endothelial cells (CEC) in febrile and polyuria phases of patients with hemorrhagic fever with renal syndrome (HFRS). Methods Expressions of CD141, CD31 and CD95 in the peripheral blood of patients with HFRS in febrile and polyuria phases were detected by flow cytometry. Comparisons among groups were done by one-factor analysis of variance. Results The percentages of CD141+ CD31+ cells in the peripheral blood cells from patients with HFRS in febrile and polyuria phases were 9.47% ±1.98 % and 8. 26% ±1.55 %, respectively, which were both higher than that (7.05%±1.45%) in healthy controls (F=8. 42; P=0. 000 and P=0. 029, respectively), and that in febrile phase was higher than that in polyuria phase (P = 0. 048). The mean fluorescent intensity (MFI) of CD95 on CEC of HFRS patients in febrile and polyuria phases were both significantly higher than that in healthy controls (F=19. 93; P=0. 000 and P=0. 000 respectively), and that in febrile phase was higher than that in polyuria phase (P=0. 049). In the febrile phase of HFRS,the MFI of CD95+ on CEC in patients with all clinical types were all higher than that in healthy controls (F= 17. 36; all P=0. 000), and that in severe (critical) type was the highest and higher than those in mild type and moderate type (P=0. 002 and P=0. 009, respectively). Conclusion The proportion of CEC and expression of CD95 on CEC are possibly related with the phase and severity of HFRS.