两种生测法检测杀虫剂对家蝇毒力的比较研究

目的用两种生物检测方法--点滴法与喂饲法检测杀虫剂对家蝇的毒力,比较这两种方法检测结果的差异.方法采用点滴法和喂饲法检测3种类型杀虫剂对两种品系家蝇的毒力.结果Lab品系差异较少,为0.48～1.69倍（LD50/LC50）;Cyh-R品系差异较大,为0.71～87.13倍（LD50/LC50）,抗药性品系的雄蝇差异较小.结论对于敏感品系家蝇可用点滴法或喂饲法检测杀虫剂的毒力,其结果差异不大;对于抗药性品系由于复杂的抗药性机制,两种方法不能替代;对于用做毒饵制剂的杀虫剂,建议用喂饲法进行检测毒力和抗药性研究.

Compared Studies on Test of Insecticides Toxicity to Musca domestica with Two Biological Methods

Objective To compare the efficiency of insecticides toxicity to different houseflies strains with two methods.Methods Using topical application method and feeding test method to test the efficiency of insecticide toxicity to different houseflies strains.Results To laboratory sensitive strains(Lab),the ratio of the topical application bioassay comparing with the feeding bioassay ranged from(0.48) to(1.69) at LD_(50)/LC_(50).To Lamda-cyhalothrin resisitant strains(Cyh-R),the ratio ranged from(0.71) to(87.13) at LD_(50)/LC_(50),for male houseflies of Cyh-R strains the difference was relatively small.Conclusion For susceptible housefly strains,the insecticide toxicity can be tested using topical application method or feeding test method, the results is similar.But for resistance strains,two methods should be used, especially for insecticide for baits formulations.