| PARP-1抑制剂对骨肉瘤细胞增殖及凋亡的影响 |
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| 引用本文： | 郑亚东,彭飞,徐西强,虞冀哲,吴华.PARP-1抑制剂对骨肉瘤细胞增殖及凋亡的影响[J].华中医学杂志,2011(1):13-16. |
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| 作者姓名： | 郑亚东 彭飞 徐西强 虞冀哲 吴华 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院骨科; |
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| 摘 要： | 目的探讨PARP-1抑制剂3-aminobenzamide（3-AB）对骨肉瘤U2OS细胞增殖及凋亡的影响。方法常规培养骨肉瘤U2OS细胞,采用CCK-8法检测U2OS细胞的增殖和流式细胞术检测细胞凋亡,并用caspase-3活性测定试剂盒检测caspase-3活性和Western-blot技术检测Bcl-2、Bax的表达。结果 3-AB能抑制骨肉瘤细胞的增殖,且与剂量和时间呈相关性,流式细胞术检测结果显示3-AB能促进骨肉瘤细胞的凋亡,且呈时间相关性,3-AB刺激早期caspase-3活性增高,Western-blot显示3-AB刺激后随时间延长Bax的表达量逐渐升高,而Bcl-2的表达量则轻度升高后降低。结论 PARP-1抑制剂3-AB能抑制骨肉瘤细胞的增殖,促进其凋亡,这为PARP-1作为骨肉瘤治疗的新靶点提供了初步实验依据。
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| 关 键 词： | 骨肉瘤 细胞增殖 细胞凋亡 |
Inhibitory effects of PARP-1 inhibitor on growth of human osteosarcoma cells. |
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| ZHENG Ya-dong,PENG Fei,XU Xi-qiang,et al..Inhibitory effects of PARP-1 inhibitor on growth of human osteosarcoma cells.[J].Central China Medical Journal,2011(1):13-16. |
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| Authors: | ZHENG Ya-dong PENG Fei XU Xi-qiang |
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| Institution: | ZHENG Ya-dong,PENG Fei,XU Xi-qiang,et al.Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the influence of PARP-1 inhibitor(3-aminobenzamide,3-AB) on the proliferation and apoptosis of osteosarcoma cells.Methods The U2OS cells were routinely cultured.CCK-8 was used to analyze cell proliferation,apoptosis was assessed by flow cytometry,the caspase-3 activity was measured by caspase-3 colorimetric assay kit,and the expression of Bcl-2 and Bax was detected by using Western blot.Results 3-AB suppressed the proliferation of osteosarcoma cells in a dose-and time-dependent mann... |
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| Keywords: | Osteosarcoma Cell proliferation Apoptosis |
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