含载脂蛋白B的脂蛋白对小鼠腹腔巨噬细胞内脂质的影响

将小鼠腹腔巨噬细胞和含载脂蛋白Ｂ的两种脂蛋白、这两种脂蛋白与硫酸葡聚糖或相应抗体形成的复合物一起培养２４ｈ后，用薄层层析法测定巨噬细胞内胆固醇酯的含量。结果发现，天然脂蛋白（ａ）对巨噬细胞内胆固醇酯含量影响不大（增加８％，Ｐ〉０．０５）；天然低密度脂蛋白具有促进巨噬细胞内脂质蓄积作用（增加３５．４％，Ｐ〈０．０５）；上述两种脂蛋白与硫酸葡聚糖一起共同作用，促进巨噬细胞内胆醇酯蓄积作用明显加强（分别

The Effect of Apolipoprotein B-containing Lipoproteins on Lipids Content in Mouse Peritoneal Macrophages

Macrophages were incubated with apolipoprotein B-containing lipoproteins, the complexes of lipoprotein with dextran sulfate (DS) or antibodies (lipoprotein cholesterol 200 mg·L~(-1), dextran sulfate 0.4 mg or antibodies 0.1 ml) for 24 h. The cholesteryl esters (CE) in macrophages were determined by thinlayer chromatography. The results that native lipoprotein (a) did not give rise to any significant storage of intracellular cholesteryl esters (0. 9 mg·g~(-1) cell protein,p>0. 05); low density lipoprotein (LDL)increased CE formation in macrophages ( 4.0 mg·g~(-1) cell protein, p<0. 05); lipoprotein (a)-DS or LDL-DS complexes caused a significant enhance in CE formation in macrophages, which were 7. 7 ,and 8. 7 mg·g~(-1) cell protein ,respectively (P < 0. 01 ); similar result were observed when macrophages were incubated with both the complexes of lipoprotein (a) with antibodies against apolipoprotein (a) or apolipoprotein B and LDL with antibodies against apolipoprotein B,which were 19.9, 18.3 and 17. 4 mg·g~(-1) cell protein, respectively (p<0. 01 ) . The above data showed that the complexes of apolipoprotein B-containing lipoproteins with DS or antibodies could cause lipid accumulation in macrophages.