2012 - 2017年武汉市HIV抗体确证检测结果分析

目的 对2012 - 2017年武汉市HIV初筛阳性样本的检测结果进行分析，了解武汉市HIV抗体确证情况。方法 依据《全国艾滋病检测技术规范（2009年版、2015年修订版）》对各筛查机构HIV筛查阳性反应的样本进行酶联免疫复核和免疫印迹确证试验。结果 2012 - 2017年武汉市艾滋病确证实验室共收到11 749份初筛阳性样本，经复核和确证试验，7 433份（63.26%）诊断为HIV - 1抗体阳性，3 780份（32.17%）为HIV - 1抗体阴性，536份（4.56%）为HIV - 1抗体不确定。阳性病例最多的年龄段为16 ～30岁。536例不确定样本中有156例（30.41%）进行了随访，120例（76.92%）确证为阳性，13例（8.33%）确证为阴性，23例（14.74%）仍为不确定。结论 2012 - 2017年武汉市HIV - 1抗体阳性数增长趋势明显，应进一步加强急性期和不确定结果病人的管理，做到早发现、早治疗。

Analysis on the results of HIV antibody confirmatory in Wuhan from 2012 to 2017

Objective To understand the consequence of HIV antibody confirmatory testing among the suspected HIV positive samples collected in Wuhan from 2012 to 2017. Methods According to the National Guideline for Detection of HIV/AIDS in China (2009 and 2015), the HIV suspected samples were screened by two fourth-generation HIV enzyme immunoassays for HIV p24 antigen and antibodies to HIV-1 and HIV-2, and specimens with at least one reactive result were further confirmed with HIV-1/2 BLOT 2.2 WB kit. Results A total of 11749 specimens were collected from 2012 to 2017, of which 7433 (63.26%) were confirmed to be HIV-1 antibody reactive, 3780(32.17%) were non-reactive and 536(4.56%) were indeterminate. As the sample number kept increasing during study period, the numbers of reactive samples in each year were stably increasing, yet the detection rates for HIV-Ab decreased. Among 536 subjects with HIV indeterminate results, only 156(30.41%) were followed up with HIV detection, of which 120 (76.92%) converted to be HIV-1 antibody positive, 13 (8.33%) were non-reactive and 23 (14.74%) remained to be HIV-1 indeterminate. Conclusion The number of HIV-infected cases in Wuhan was increasing in recent years. Specifically, a large number of acute HIV infections shown as HIV-1 antibody non-reactive or indeterminate were found, which requires further attention in the future.