锌对邻苯二甲酸酯类暴露致雄性生殖毒性的干预作用

目的：研究锌对邻苯二甲酸酯类（PEs）暴露致雄性小鼠生殖毒性的干预作用。方法：36只雄性ICR小鼠随机分对照组，邻苯二甲酸丁苄酯（BBP）、邻苯二甲酸正二丁酯（DBP）、邻苯二甲酸二（2-乙基己基）酯（DEHP）3种邻苯二甲酸酯类（PEs）等毒性混合物（MIXPs）暴露组（MIXPs组）以及MIXPs+ZnSO4组。MIXPs组小鼠经口灌胃MIXPs 160 mg·kg-1·d-1，MIXPs+ZnSO4组小鼠同时经口灌胃MIXPs 160 mg·kg-1·d-1+ZnSO4 50 mg·kg-1·d-1，对照组小鼠灌胃等容量的赋形剂（0.5% CMC-Na）。连续干预90 d，眶静脉取血后、取睾丸和附睾等雄性生殖器官，观察精子形态，试剂盒测定睾酮、SOD、MDA、T-AOC等水平。结果：与对照组相比，MIXPs组小鼠体质量显著降低，肛殖距缩短（均P＜0.05）；血清睾酮水平显著降低，精子畸形率显著升高，睾丸组织中SOD、MDA显著改变（均P＜0.01）；而MIXPs+ZnSO4组与对照组差异无统计学意义（P＞0.05）。结论：锌可抑制MIXPs暴露诱导小鼠雄性生殖毒性，可能与其改善睾丸组织的抗氧化水平，提高血清睾酮水平有关。

Intervention of zinc on the male reproductive toxicity caused by phthalates exposure

Objective: To study the protective effect of zinc on reproductive toxicity caused by phthalate exposure in mice. Methods: Thirty-six male ICR mice were randomly divided into the control group, MIXPs (the equipotent toxic mixture of butyl benzyl phthalate (BBP), di (n-butyl) phthalate (DBP) and di (2-ethyhexyl) phthalate (DEHP) group, and MIXPs+ZnSO4 group, with 12 mice in each group. Mice in MIXPs group and MIXPs+ZnSO4 group were treated with 160 mg·kg-1·d-1 MIXPs by gavage with or without 50 mg·kg-1·d-1 ZnSO4. Mice in control group were treated with isodose 0.5% CMC-Na. After 90 days’ intervention, orbital vein blood was collected from each anesthetic mouse. Then dissect the mouse and take male reproductive organs such as the testicles and epididymis. Sperm morphology was observed with electron microscope. The levels of testosterone, SOD, MDA and T-AOC were measured with the commercial kits. Results: Compared with the control, the bodyweight, anogenital distance, serum testosterone, SOD and MDA in testicular tissue in MIXPs group were significantly reduced, while the rate of sperm deformation was significantly increased (P<0.05 or P<0.01), but there were no significant changes in MIXPs+ZnSO4 group. Conclusion: Zinc can inhibit the male reproductive toxicity induced by MIXPs exposure, which may be related to the improvement of the antioxidant level of testicular tissue and the increase of serum testosterone level.