黄芪总皂苷调节环状RNA34116表达阻止肝纤维化进展

目的：探讨黄芪总皂苷（AST）对肝纤维化模型的干预作用，并从环状RNA（circRNA）的角度分析其作用机制。方法：小鼠随机分为正常组、CCl4模型组、CCl4+AST组、CCl4+索拉菲尼组，每组8只。制备CCl4小鼠肝纤维化模型，给予AST和索拉菲尼干预后，用生化试剂盒检测血清肝功能，制作石蜡切片观察肝组织病理学变化（HE染色、Masson染色），RT-PCR检测各组环状RNA34116（mmu_circ_34116）表达变化。结果：组织病理检测显示，与正常组比较，CCl4模型组肝组织炎症反应和胶原表达显著增加；AST、索拉菲尼干预后，肝组织炎症显著减轻，胶原表达显著减少（P＜0.05）；血清肝功能检测显示，与正常组比较，CCl4模型组血清ALT、TBIL水平显著上升（P＜0.01）；AST、索拉菲尼干预后ALT、TBIL水平显著下降（P＜0.01）；RT-PCR检测结果显示，与正常组比较，CCl4模型组肝组织mmu_circ_34116表达量显著降低（P＜0.01）；AST干预可显著增加mmu_circ_34116的表达量（P＜0.01）；而索拉菲尼对mmu_circ_34116的表达量无显著影响（P＞0.05）。结论：AST显著阻止肝纤维化模型的肝纤维化进展，其机制可能与调节mmu_circ_34116表达有关。

Study of anti-liver fibrosis effect of astralosides via circular RNA34116

Objective: To study the mechanism underlying Astragalosides in the inhibition of hepatic fibrosis via circularRNA. Methods: Mice were randomly divided as normal group, CCl4 model group, Astragalosides group and sorafenib group, with 8 mice in each group. A CCl4 liver fibrosis model was established. After drug intervention with Astragalosides, the serum ALT and TBIL, pathological changes (HE staining and Masson staining) of the liver and relative mmu_circ_34116 expressionwere detected and observed by RT-PCR. Results: Histopathological results showed that, compared with the normal group, liver tissue inflammation and collagen deposition were significantly increased Astragalosides and sorafenib significantly reduced inflammation and collagen deposition. The results of serum liver function showed that Astragalosides and sorafenib significantly reduced the level of ALT and TBIL after intervention in the model mice. RT-PCR results showed that, compared with the normal group, the expression of mmu_circ_34116 in liver tissue was significantly reduced in CCl4 model group, and its expression was significantly increased after intervention with Astragalosides in the model, but sorafenib had no significant effect on its expression. Conclusion: Astragalosides significantly inhibited the development of liver fibrosis. The mechanism may be associated with over expression of mmu_circ_34116.