SRC1在胃癌中的表达及其与预后的关系

目的 探讨类固醇受体辅助活化因子-1(Steroid receptor coactivator 1,SRC1)蛋白在胃癌组织中的表达及其与患者临床病理参数和预后的相关性。方法 收集36例胃癌组织及其配对的癌旁组织,qRT-PCR法及Western blot法检测SRC1mRNA和蛋白的表达水平。应用免疫组化方法检测286例胃癌组织中SRC1蛋白的表达情况,分析SRC1蛋白表达与胃癌临床病理参数的关系及其对患者预后的影响。结果 与癌旁组织相比,胃癌组织中SRC1mRNA表达水平明显降低(P=0.004),SRC1蛋白表达水平也明显降低。SRC1蛋白的表达强度与胃癌患者临床病理参数无显著关系。Kaplan-Meier生存曲线分析发现高表达SRC1组5年生存率显著高于低表达组(P=0.009)。Cox回归分析结果显示低表达SRC1(P=0.002)、肿瘤侵袭T_4a~T_4b(P=0.004)、淋巴结转移N(P=0.038)、远处转移M1(P＜0.001)是独立影响总生存时间的预后不良因素。SRC1低表达较高表达患者预后差。结论 胃癌中SRC1的表达明显降低,胃癌组织中的SRC1表达水平可作为独立的胃癌预后不良因素,其过低表达与胃癌的发展密切相关,可能作为一个抑癌基因及判断胃癌患者预后的标志物。

Expression of SRC1 in gastric cancer and its prognosis

Objective The Objective of this study was to investigate the expression of SRC1 protein in gastric cancer,and its correlation with clinicopathological parameters and prognosis.Methods Thirty-six gastric cancer tissues and matched paracancerous tissues were collected.The expressions of SRC1 at levels of mRNA and protein were detected by qRT-PCR and Western blot.The expression of SRC1 protein in 286 cases of gastric cancer was detected by immunohistochemistry.The relationship between the expression of SRC1 protein and the clinicopathologic parameters of gastric cancer and the prognosis of the patients were analyzed.Results The expression of SRC1 at levels of mRNA and protein were significantly lower in gastric cancer tissues than that in the normal gastric tissues(P=0.004).There was no significant correlation between the expression of SRC1 protein in gastric cancer tissues and the clinicopathological parameters of gastric cancer patients.Kaplan-Meier survival curve analysis showed that the 5-year survival rate of SRC1 overexpressing group was significantly higher than that in the low expression group(P=0.009).Cox regression analysis showed that the low expression of SRC1,tumor invasion of T_4a~T_4b,lymph node metastasis N,and distant metastasis M1 were independent prognosis predictors of total survival time(P＜0.05 or P＜0.001).The low expression of SRC1 protein was associated with poor prognosis in gastric cancer.Conclusion The expression of SRC1 protein is low in gastric cancer.The SRC1 expression may be an independent prognostic factor for gastric cancer.The overexpression of SRC1 may be related to the development of gastric cancer,which may serve as a marker of tumor suppressor gene and prognosis of gastric cancer patients.