RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体的影响

目的:利用RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体构成比的影响。方法:临床选择40例中重度慢性牙周炎患者,随机分成CPC(试验)组、溶媒(对照)组,牙周基础治疗后分别使用该药品含漱+袋内冲洗。记录患者基线和用药4周后的临床指标;采集龈下菌斑、龈沟液和唾液样本,BANA试验检测胰蛋白酶样酶含量,RT-qPCR检测P.gingivalis、T.forsythia、T.denticola在总菌量中的构成比。结果:治疗后:1)试验组AL、BOP、PLI显著改善(P<0.01),对照组仅AL改善(P<0.01);2)两组龈下菌斑和龈沟液样本中胰蛋白酶样酶含量均显著下降(P<0.05);3)两组P.gingivalis构成比均显著下降(P<0.01),T.forsythia在试验组构成比显著下降(P<0.01),而T.denticola治疗前后无变化;4)P.gingivalis、T.forsythia、T.denticola两两之间构成比均显著相关(P<0.01)。结论:CPC可抑制牙菌斑形成,改善患者的临床症状,并对龈下菌斑中的P.gingivalis有一定的抑制作用,临床上可用于辅助治疗中重度慢性牙周炎。

Effect of Cetylpyridinium Chloride on Red Complex Bacteria in Moderate to Severe Chronic Periodontitis Patients by Real-time Quantitative PCR

Objective: To determine the effect of CPC mouthwash on the proportions of red complex bacteria in patients with moderate to severe chronic periodontitis by RT-qPCR. Methods: Forty patients with periodontitis were randomly assigned to two groups: experimental group (SRP+CPC) and control group (SRP+ placebo). Clinical parameters were recorded at baseline and at 4 weeks. Subgingival plaque, gingival crevicular fluid, and saliva samples were collected, and the content of trypsin-like enzyme was detected using BANA test. RT-qPCR was used to detect the proportions of P.gingivalis, T.forsythia and T.denticola in total bacteria. Results: AL, BOP, and PLI significantly improved (P<0.01) after treatment in the experimental group. Only AL significantly improved in the control group (P<0.01). The level of trypsin-like enzyme in subgingival plaque and gingival crevicular fluid for both groups decreased significantly (P<0.05). The numbers of P. gingivalis decreased significantly in both groups (P<0.01), while T.forsythia decreased significantly in the experimental group (P<0.01). There was no significant difference in the numbers of T.denticola after treatment compared to baseline. There was a significant correlation between the proportions of P.gingivalis, T.forsythia, and T.denticola (P<0.01). Conclusion: CPC can inhibit plaque formation and improve clinical parameters in patients with moderate to severe chronic periodontitis, but has no significant inhibitory effect on P.gingivalis, T.forsythia, and T.denticola.