人体距骨显微硬度的分布特征研究

目的 探讨人体距骨显微硬度的分布特征。方法 自河北医科大学解剖教研室标本库中,纳入年龄＞40岁、既往身体健康、无慢性病史的新鲜冰冻尸体标本3具,取其右侧距骨。以距骨颈基底部为界将距骨分为距骨头颈和距骨体两部分,并将距骨头颈部纵向平分为内、外两部分,将距骨体分别沿横向和纵向平分为前、后两部分和内、外两部分。用慢速锯沿距骨长轴进行切割,将骨骼制成若干厚3 mm的骨组织切片,并用砂纸打磨。应用维氏方法测量骨切片骨皮质和骨松质不同区域的硬度值。采用50 g力加载50 s、维持12 s标准操作方法测定,同一区域选取5个有效值,全体有效值的平均值作为该部位的硬度值。结果 本研究共制备18个骨组织切片,测量180个位点。三具标本距骨总体骨硬度为(36.70±5.77)HV。距骨头颈部硬度为(37.68±5.22) HV,距骨体硬度为(36.20±5.99)HV,两者比较差异无统计学意义(t=1.624, P＞0.05);距骨头颈部内侧硬度为(38.60±5.17) HV,略高于其外侧的(36.76±5.19) HV,但差异无统计学意义(t=1.376, P＞0.05)。距骨体内侧硬度为(37.78±5.23) HV,高于外侧的(34.63±6.32) HV ,差异有统计学意义(t=2.971, P＜0.01);距骨体前、后两部位硬度值分别为(36.38±5.50) HV和(36.03±6.49) HV,差异无统计学意义(t=0.375, P＞0.05)。结论 本研究首次报道应用维氏硬度方法分析人体距骨维氏硬度的分布规律:距骨头颈部与体部硬度无明显差异,距骨体内侧骨硬度大于外侧,是匹配内侧的高应力的结果,符合人体正常生理功能。

The micro-hardness distribution in human talus bone

Objective To explore the distribution of microhardness of human talus bone.Methods All the talus bones included in this study were collected from the Anatomy Department of Hebei Medical University, and 3 fresh frozen corpses aged above 40 years old, no chronic medical history were selected. All the right talus was taken and the soft tissue was dissected. With the base of the talus neck as the boundary, the talus was divided into the talus head-neck and the talus body using a slow-speed saw. Both the talus head-neck and talus body were bisected into the medial and lateral parts along the longitudinal axis and then the talus body was bisected into the anterior and posterior parts along the transverse axis. The bones were made into several 3 mm thick bone tissue sections and sanded with sandpaper. A microindenter fitted with a Vickers indenter point was used to measure the Vickers hardness in cortical bone and trabecular bone in different regions. The indentation load and dwell time was set to 50 g and 12 s for both the cortical and cancellous tissues in this study. For each site or region, 5 valid values were recorded and averaged as the Vickers hardness for this site or region.Results Totally, 18 bone tissue sections and 180 indentations at different bones and anatomic sites were involved in our result. The overall bone Vickers hardness of the talus bone was (36.70±5.77)HV, including (37.68±5.22) HV in the talus head-neck and (36.20±5.99) HV in the talus body. The Vickers hardness of the talus head-neck was similar to the talus body(t=1.624, P＞0.05). There was no significant difference in the Vickers hardness between the medial[(38.60±5.17)HV] and lateral site[(36.76±5.19) HV] of the talus head-neck(t=1.376, P=0.174). Whereas in the talus body, the Vickers hardness in the medial site[(37.78±5.23)HV] of the talus body was higher than that of the lateral site[(34.63±6.32)HV](t=2.971, P＜0.01), and the Vickers hardness in the anterior and posterior parts of talus body were(36.38±5.50) HV and (36.03±6.49) HV(t=0.375, P＞0.05).Conclusions This study reports for the first time the distribution of Vickers hardness of human talus bone. There was no significant difference in the Vickers hardness between the talus head-neck and talus body. The Vickers hardness in medial site of the talus body is higher than that of the lateral site, matching the high stress on the medial site of the talus body, which were eligible to physiological function of human body.