芹菜叶对自发性高血压大鼠内皮型一氧化氮合酶及长链非编码RNA-sONE的影响研究

背景　高血压是心血管疾病和全因死亡的重要危险因素。研究表明，长链非编码RNA（LncRNA）的异常表达与心血管疾病的发生密切相关。目的　探讨芹菜叶对自发性高血压大鼠内皮型一氧化氮合酶（eNOS）、LncRNA-sONE的影响。方法　2015年9月，将购买的48只（雌雄各24只）10周龄、SPF级、自发性高血压大鼠正常饮食（0.25%的NaCl饲料）饲养2周后，随机分为高盐组、正常盐组和高盐芹菜叶组，每组16只（雌雄各半）。高盐组给予5.00%的NaCl饲料饲养12周；正常盐组给予0.25%的NaCl饲料饲养12周；高盐芹菜叶组先予5.00%的NaCl饲料饲养8周后，再予5.00%的NaCl饲料+10.00%芹菜叶饲养4周；此外，各组予以足量其他饮食。比较3组大鼠干预前及干预4周、8周、12周体质量、收缩压。采用Western blotting法检测eNOS蛋白表达水平，qPCR法检测LncRNA-sONE、eNOS mRNA表达水平。结果　高盐组、高盐芹菜叶组大鼠干预8周、12周体质量均低于正常盐组（P<0.05）。高盐组、高盐芹菜叶组大鼠干预4周、8周、12周收缩压高于正常盐组（P<0.05）；高盐芹菜叶组大鼠干预12周收缩压低于高盐组（P<0.05）。高盐组大鼠干预12周eNOS蛋白表达水平低于正常盐组，LncRNA-sONE、eNOS mRNA表达水平高于正常盐组（P<0.05）；高盐芹菜叶组大鼠干预12周eNOS蛋白、eNOS mRNA表达水平高于正常盐组（P<0.05）；高盐芹菜叶组大鼠干预12周eNOS蛋白、eNOS mRNA表达水平高于高盐组，LncRNA-sONE表达水平低于高盐组（P<0.05）。结论　芹菜叶能升高自发性高血压大鼠eNOS蛋白及其mRNA表达水平，降低LncRNA-sONE表达水平；同时eNOS、LncRNA-sONE可能参与了芹菜叶的降压机制。

Effects of Celery Leaves on Endothelial Nitric Oxide Synthase and Long Non-coding RNA-sONE in Spontaneously Hypertensive Rats

Background　Hypertension is an important risk factor for cardiovascular disease and all-cause death.Previous studies have shown that the abnormal expression of long non-coding RNA （LncRNA） is closely related to the occurrence of cardiovascular diseases.Objective　To investigate the effects of celery leaves on endothelial nitric oxide synthase （eNOS） and LncRNA-sONE in spontaneously hypertensive rats.Methods　In September 2015，48 spontaneously hypertensive rats （10 weeks old，SPF grade，half males and half females） were purchased.After being fed with normal diet （forage containing 0.25% NaCl） for 2 weeks，they were randomly and evenly divided into high salt group，normal salt group and high salt celery leaf group，with balanced sex ratio，receiving another 12-week diet intervention with forage containing 5.00% NaCl，12-week diet intervention with forage containing 0.25% NaCl，and 8-week synthetic forage containing 5.00% NaCl and 10.00% celery leaves，respectively.In addition，each group was given adequate other diets.Body weight and systolic blood pressure（SBP） of three groups were measured and compared before intervention，and after 4，8 and 12 weeks of intervention.Western blotting was used to detect the expression of eNOS protein.qPCR was used to detect the expression of LncRNA-sONE and eNOS mRNA.Results　Normal salt group showed higher body weight than other two groups after 8 and 12 weeks of intervention （P<0.05）．Normal salt group demonstrated lower SBP than other two groups after 4，8 and 12 weeks of intervention （P<0.05）．High salt celery leaf group had lower SBP than high salt group after 12 weeks of intervention （P<0.05）．By the end of the 12th week of intervention，compared with normal salt group，high salt group showed lower expression level of eNOS protein but higher expression levels of LncRNA-sONE and eNOS mRNA（P<0.05），high salt celery leaf group showed higher expression levels of eNOS protein and eNOS mRNA（P<0.05）．High salt celery leaf group demonstrated higher expression levels of eNOS protein and eNOS mRNA and lower expression level of LncRNA-sONE when the intervention ended compared with high salt group （P<0.05）．Conclusion　Celery leaves could increase the expression levels of eNOS protein and mRNA，and decrease the expression level of LncRNA-sONE in spontaneously hypertensive rats.In addition，eNOS and LncRNA-sONE may be involved in the antihypertensive mechanism of celery leaves.