野葛主要成分积累动态变化研究

 目的通过对野葛块根产量和主要活性物质含量积累动态变化规律进行研究,以确定野葛的适宜采收期。方法用紫外法于250 nm处比色以测定总异黄酮含量和HPLC测定其葛根素含量。结果5年生野葛6月份采收,葛根素和异黄酮含量最高,分别为7.91%,8.73%;11月份二者含量次之,分别为5.90%,7.53%。而块根产量在11月份最高,且单株块根中葛根素和异黄酮总量最高,分别为29.81,38.05g。结论5年生野葛在11月采收具有最高的利用价值。

Study on Dynamic Accumulation of Main Components in Pueraria lobata (Willd) Ohwi.

OBJECTIVE To study the optimal harvesting time of Pueraria lobata (Willd) Ohwi.by determining the contents of puerarin and isoflavone in the root of plants in different growth duration and by the weight of the root.METHODS The contents of puerarin and isoflavone were determined by HPLC-UV assay.The detection wavelength was at 250 nm.RESULTS 5-Year old Pueraria lobata(Willd)Ohwi was gathered in June.The maximum contents of puerarin and isoflavone were obtained,at 7.91% and 8.73% ,respectively.In november,the contents were 5.90% and 7.53%.However,the weight of root was highest in november,the maxiumu total yields of puerarin and isoflavone were obtained in november,and the yields were 29.81 g and 38.05 g.CONCLUSION The root of 5-year old Pueraria lobata(Willd) Ohwi gathered in November was suitable to harvest for medicine.