人胚神经干细胞在大鼠脊髓内的分化研究

目的　人胚神经干细胞 (neuralstemcellsNSCs)经全反式维甲酸 (retinoicacid)预处理后移植入大鼠脊髓内 ,观察细胞的分化情况。方法　分离 10周左右流产人胚皮层组织中的神经干细胞 ,进行体外增殖 ,部分细胞在移植前 6d加入全反式维甲酸共培养。SD大鼠 3 0只 ,随机分为 2组 :实验组移植经维甲酸预处理后的NSCs ;对照组移植未经维甲酸预处理后的NSCs。移植后 5周取出脊髓 ,应用免疫组化技术检测Brd U标记的NSCs在大鼠脊髓内的生存和分化情况。结果　大鼠脊髓内可见大量Brd U阳性细胞 ,部分能够分裂增殖 ,向注射点远处迁移。实验组的NSCs能分化出NF、GFAP、Gal c阳性细胞 ;对照组的NSCs不能分化出NF阳性细胞。结论　人胚NSCs可在宿主脊髓内生存、分裂、迁移 ;经维甲酸预处理后的NSCs能分化成神经元和神经胶质细胞 ;人胚神经干细胞可作为 1种理想的移植材料 ,而具有重要临床应用价值

DIFFERENTIATION OF NEURAL STEM CELLS FROM HUMAN EMBRYOS IN THE RATSPINAL CORD

Objective: To observe the differentiation of neural stem cells(NSCs) from human embryos with retinoic acid pretreatment in the rat spinal cord. Methods: Proliferation of undifferentiated neural stem cells (NSCs) were isolated from the embryonic human cerebral cortex of abant 10 weeks. Parts of the cells were exposed to retinoic acid for 6 before transplantation. Thirty adult SD rats were used and randomly divided into two groups: ① In the experimental group, the NSCs were transplanted with retinoic acid pretreatment into the rat spinal cord; ②In the control group, the NSCs were transplanted without retinoic acid pretreatment. 5 weeks later histological analysis showed the differentiation of Brd-U-labelled cells. Result: There are many Brd-U-labelled cells in the rat spinal cord, some of which can proliferate and migrate away from the injection site. The cells exposed to retinoic acid can express the astrocytic maker-GFAP, oligodendrocytic maker-Gal-c, and neuronal maker-NF; The cells un exposed to retinoic acid cannot express the neuronal maker-NF. Conclusions: The transplanted NSCs survive, proliferate and migrate away from the injection site. The cells with retinoic acid pretreatment can differentiate into neurons. Therefore, as an ideal source of transplantation, NSCs may play a great role in the later clinical practice.