| 毛细管气相色谱法测定人体血液中的脂肪酸 |
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| 引用本文： | 张伟利 Grue.,A.毛细管气相色谱法测定人体血液中的脂肪酸[J].上海交通大学学报(医学版),1997,17(5):350-353. |
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| 作者姓名： | 张伟利 Grue. A |
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| 摘 要： | 介绍采用石英毛细管气相色谱法测量人体血液中的游离脂肪酸（FFA）和总脂肪酸（TFA）。该方法灵敏，可分离血浆中近20种脂肪酸，其中油酸（C18:1）、软脂酸（C16：0）、亚油酸（C18：2）、硬脂酸（C18：0）和软脂油酸（C16：1）5种脂肪酸占了血浆中FFA总量的约90％。本方法所需血量少，更适用于采血较困难的婴幼儿进行脂肪营养的研究。
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| 关 键 词： | 毛细管柱 色谱法 脂肪酸 |
Analysis of Plasma Free Fatty Acids and Total Fatty Acids by Capillary Gas Chromatography |
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| Zhang Weili, Adolf Grnert.Analysis of Plasma Free Fatty Acids and Total Fatty Acids by Capillary Gas Chromatography[J].Journal of Shanghai Jiaotong University:Medical Science,1997,17(5):350-353. |
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| Authors: | Zhang Weili Adolf Grnert |
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| Abstract: | Present report describes a simple method to analyze free fatty acid (FFA) and total fatty acid (TFA) contents in human plasma by gas chromatography. About 20 fatty acids were separated on a capillary column with a SP- 2330 stationary phase. The five fatty acids, oleic (18:1w9),palmitic (16:0), linoleic (18:2w6), stearic (18:0) and palmitoleic (16:1) acids account for about 90 percent of plasma FFA. As little as 0.2ml of plasma is used for the analyses, so this method is suitable for determining the blood fatty acids in small infants. |
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| Keywords: | capillary column gas chromatography fatty acids |
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