抗CD4及抗CXCR4抗体阻断HIV—1感染细胞作用的研究

为探讨抗ＣＤ４ 及抗ＣＸＣＲ４ 抗体在阻断Ｉ型人免疫缺陷病毒（ＨＩＶ－ １） 感染应用中的意义， 本文应用上述两种抗体分别与ＳｕｐＴ１ 细胞及人外周血单个核细胞（ＰＢＭＣ） 共培育， 以封闭ＨＩＶ－ １ 在上述细胞上的受体。然后， 以ＨＩＶ１ ＮＬ４３ 病毒株感染上述细胞， 通过测定感染细胞上清中ＨＩＶ－ １ 的Ｐ２４ 蛋白含量， 观察上述抗体对ＨＩＶ－ １ 感染细胞的阻断作用。结果显示， 无论是抗ＣＤ４ 或抗ＣＸＣＲ４ 的抗体单独应用或是两者联合应用， 均可明显地抑制ＨＩＶ－ １ 感染细胞的作用。该结果为今后开拓ＡＩＤＳ的抗体治疗提供了理论基础。

Blocking HIV-1 infection in SupT1 and peripheral blood mononuclear cells by anti-CD4 and anti-CXCR4 antibodies

To evaluate the potential use of anti CD4 and anti CXCR4 antibodies for the blocking of human immunodeficiency virus type I(HIV 1)infection, SupT1 cells and peripheral blood mononuclear cells(PBMC) were challlenged with NL4 3 virus at the MOIs=0 04 following the cells co incubated with the antibodies for 2 hours and then the p24 antigen levels in the supernatant from challenged cells were determined at inter vals of 4 days after infection The results showed that both anti CD4 and anti CXCR4 antibodies can significantly block HIV 1 infection of SupT1 cells or PBMCs and that these antibodies may have potential useful values in the prevention or treatment of HIV 1 infection