| 三种抗G—CSF单链抗体基因的表达及活性鉴定 |
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| 引用本文： | 贾松惠,郭鹞.三种抗G—CSF单链抗体基因的表达及活性鉴定[J].细胞与分子免疫学杂志,1999,15(4):256-258. |
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| 作者姓名： | 贾松惠 郭鹞 |
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| 作者单位： | 第四军医大学病理生理学教研室!西安710032(贾松惠)，第四军医大学!西安710032(郭鹞)，中国预防医学科学院病毒基因工程国家重点实验室!北京100052(范灵芝，梁米芳，侯云德) |
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| 摘 要： | 从重组人ＧＣＳＦ免疫小鼠全套单链抗体噬菌体表面展示文库中， 筛选到３ 个具有ＧＣＳＦ抗原结合活性的重组噬菌体单链抗体基因。将其感染不含Ｓｕｐ Ｅ的Ｅ． ｃｏｌｉ 菌株， 获得以ＳｃＦｖＥｔａｇ 形式的目的抗体片段的可溶性表达。经ＥＬＩＳＡ和Ｄｏｔｂｌｏｔ 检测， 表达产物具有与ＧＣＳＦ抗原结合的活性。ＮＦＳ６０ 细胞株抑制实验初步表明， 表达产物具有抑制ＧＣＳＦ的作用。
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| 关 键 词： | G-CSF 单链抗体 基因表达 |
Expression and activity identification of three anti G CSF single chain Fv antibody genes |
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| Jia Songhui,Guo Yao ,Fan Lingzhi ,Liang Mifang ,Hou Yunde.Expression and activity identification of three anti G CSF single chain Fv antibody genes[J].Journal of Cellular and Molecular Immunology,1999,15(4):256-258. |
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| Authors: | Jia Songhui Guo Yao Fan Lingzhi Liang Mifang Hou Yunde |
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| Institution: | Jia Songhui,Guo Yao 1,Fan Lingzhi 2,Liang Mifang 2,Hou Yunde 2 |
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| Abstract: | We screened out three G CSF binding single chain Fv(ScFv) antibody genes from a phage display library of ScFv antibody repertoire derived from mouse immunized with recombinant human G CSF. And then the Sup E lacking E.coli HB2151 was infected by the positive recombinants. The soluble ScFv antibody fragments were expressed in a fusion form of ScFv E tag. The results showed that expression products could bind to G CSF by ELISA and dot blot; and inhibitory activity of G CSF by NFS 60 inhibitory assay. |
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| Keywords: | G CSF single chain Fv antibody gene expression |
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