抗空肠弯曲菌PEB1-LTB疫苗联合应用增强免疫应答的研究

目的为研制有效的空肠弯曲菌疫苗,构建PEB1与LTB融合基因的空肠弯曲菌疫苗,并初步探讨pcDNA3.1(-)-PEB1-LTB核酸疫苗与蛋白疫苗联合免疫BALB/c小鼠的免疫应答水平。方法通过腿部肌肉注射免疫小鼠的方式,在第0、3、6周采用核酸单独免疫与核酸蛋白联合免疫小鼠的免疫程序,于第5、8周末,测量小鼠体液免疫应答和细胞免疫应答水平。结果 3次免疫后,核酸蛋白免疫组诱导的IgG抗体含量(14.392±0.579)μg/ml]是核酸疫苗单独免疫的2.43倍(P<0.05),说明核酸蛋白联合免疫诱导了更高的体液免疫应答水平;3次免疫后,核酸疫苗单独免疫组诱导的IFN-γ水平(1 472.34±73.99)pg/ml]是联合免疫组(290.323±15.46)pg/ml]的5.07倍,说明PEB1-LTB核酸疫苗单独免疫诱导较高的细胞免疫应答。结论核酸疫苗能诱导较强的细胞免疫应答,与PEB1-LTB蛋白联合免疫呈现出不同的免疫应答特点。该研究为抗空肠弯曲菌疫苗的优化设计与合理应用提供了理论依据。

PEBI-LTB DNA vaccine primer-protein boost enhances the immunization against Campylobacter jejuni in mice

Objective To develop novel and effective Campylobacter jejuni vaccine, we constructed Campylobacter jejuni gene PEB1 fused LTB. In present study, we determined if the pcDNA3.1（-）-PEB1-LTB DNA vaccines boosting with PEB1-LTB protein vaccines could enhance immunization in mice. Methods We immunized mice by intramuscular injection. The mice were inoculated with DNA vaccines and DNA boosting with protein at 0, 3, 6 week. The specific humoral and cellular immune responses were detected at 5,8 week. Results In the DNA vaccine prime-boost group after 3 times, the levels of IgG in serum, （14.392±0.579）μg/ml were higher than the others. The DNA vaccine boosting protein vaccine could enhance the humoral response. But the levels of IFN-γ, （1472.34±73.99）pg/ml in PEB1-LTB DNA vaccine were the highest. Conclusions DNA vaccines can induce different immunization, specially the better cellular immune responses, compared with DNA vaccines boosting with protein vaccines. The results provide a basis for ration design and application of the Campylobacter jejuni vaccine.