| 脑源性神经营养因子转基因成纤维细胞的构建与检测 |
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| 引用本文： | 孟步亮,李力燕,徐丹,刘佳,王廷华. 脑源性神经营养因子转基因成纤维细胞的构建与检测[J]. 神经解剖学杂志, 2011, 27(1): 110-114 |
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| 作者姓名： | 孟步亮 李力燕 徐丹 刘佳 王廷华 |
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| 作者单位： | 1. 昆明医学院:人体解剖学教研室,昆明,650031
2. 昆明医学院:神经科学研究所,昆明,650031 |
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| 摘 要： | 目的:构建大鼠脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)基因修饰的成纤维细胞(fibroblasts FBs).方法:真核表达载体pcDNA3/BDNF由昆明医学院神经科学研究所提供.FBs取自胎鼠.pcDNA3/BDNF重组质粒载体以阳离子脂质体Lipofect...
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| 关 键 词： | 脑源性神经生长因子 成纤维细胞 转染 |
Construction and detection of the fibroblasts modified with brain-derived neurotrophic factor gene |
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| Meng Buliang,Li Liyan,Xu Dan,Liu Jia,Wang Tinghua. Construction and detection of the fibroblasts modified with brain-derived neurotrophic factor gene[J]. Chinese Journal of Neuroanatomy, 2011, 27(1): 110-114 |
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| Authors: | Meng Buliang Li Liyan Xu Dan Liu Jia Wang Tinghua |
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| Affiliation: | Meng Buliang1,Li Liyan2,Xu Dan2,Liu Jia2,Wang Tinghua2(1.Department of Anatomy,2.Institute of Neuroscience,Kunming Medical College,Kunming 650031,China) |
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| Abstract: | Objective:To construct the fibroblasts(FBs) mordified with brain-derived neurotrophic factor(BDNF)gene.Methods:The recombinant vector(pcDNA3/BDNF)was supplied by Institute of Neuroscience of Kunming Medical College.FBs were taken from the embryo of rats.FBs were transfected with pcDNA3/BDNF using LipofectamineTM 2000 transfection reagent.The transfection efficiency has also been statisticed.The expression of BDNF was analyzed by immunocytochemistery and Western Blot.PC12 cells cultured with the supernatant ... |
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| Keywords: | brain-derived neurotrophic factor fibroblast transfection |
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