| 替莫唑胺(TMZ)联合2-甲氧基雌二醇(2-ME)对小鼠胶质瘤化疗作用的体内实验研究 |
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| 引用本文： | 徐高峰,王茂德,姜海涛,谢江涛,谢万福,李瑞春.替莫唑胺(TMZ)联合2-甲氧基雌二醇(2-ME)对小鼠胶质瘤化疗作用的体内实验研究[J].神经解剖学杂志,2011,27(1):82-86. |
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| 作者姓名： | 徐高峰 王茂德 姜海涛 谢江涛 谢万福 李瑞春 |
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| 作者单位： | 西安交通大学医学院第一附属医院神经外科,西安,710061 |
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| 摘 要： | 目的:探讨替莫唑胺(temozolomide,TMZ)联合2-甲氧基雌二醇(2-methoxyestradiol,2-ME)对SHG-44胶质瘤的抑制作用。方法:培养SHG-44细胞,建立裸鼠荷瘤模型,荷瘤后将裸鼠随机分空白对照组、TMZ组、2-ME组、TMZ+2-ME组,每组各7只,荷瘤后8 d分别给予TMZ、2-ME、TMZ+2-ME,观察肿瘤的生长情况,给药结束5周后取瘤计算抑瘤率,并制备瘤组织细胞悬液行流式细胞仪检测细胞凋亡率,Western Blot法检测瘤组织HIF-1α、P53蛋白表达。结果:(1)TMZ组、2-ME组及2-ME+TMZ组肿瘤生长均低于空白对照组(P(0.05);2-ME+TMZ组肿瘤生长低于TMZ组。(2)2-ME+TMZ组、TMZ组及2-ME组的瘤细胞凋亡率均高于空白对照组(P(0.05),2-ME+TMZ组与TMZ组的凋亡率相比无统计学差异(P(0.05)。(3)2-ME+TMZ组HIF-1α蛋白表达明显低于TMZ组;2-ME+TMZ组P53蛋白表达明显高于TMZ组。结论:应用替莫唑胺(TMZ)联合2-甲氧基雌二醇(2-ME)对裸鼠皮下移植瘤的治疗作用优于单用TMZ或2-ME。
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| 关 键 词： | 胶质瘤 替莫唑胺 2-甲氧基雌二醇 联合用药 小鼠 |
Study of chemotherapeutic effects of temozolomide (TMZ) combined with 2-methoxyestradiol (2-ME) on gliomas in vivo in mouse |
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| Xu Gaofeng,Wang Maode,Jiang Haitao,Xie Jiangtao,Xie Wanfu,Li Ruichun.Study of chemotherapeutic effects of temozolomide (TMZ) combined with 2-methoxyestradiol (2-ME) on gliomas in vivo in mouse[J].Chinese Journal of Neuroanatomy,2011,27(1):82-86. |
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| Authors: | Xu Gaofeng Wang Maode Jiang Haitao Xie Jiangtao Xie Wanfu Li Ruichun |
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| Institution: | Xu Gaofeng,Wang Maode,Jiang Haitao,Xie Jiangtao,Xie Wanfu,Li Ruichun(Department of Neurosurgery,First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective:The purpose of this study was to compare TMZ(temozolomide) alone and TMZ plus 2-methoxyestradiol(2-ME) on gliomas' chemotherapy in the immunodeficiency mouse.Methods:Tumor xenografts in immunodeficient mouse were established and divided into 4 groups:control group,TMZ group,2-ME group and 2-ME+TMZ group.Eight days after grafting,mouse was given TMZ,2-ME,TMZ +2-ME respectively.Mouse was observed for appearance of subcutaneous nodules at injection site.Five weeks after treatment,mouse were killed an... |
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| Keywords: | glioma temozolomide 2-methoxyestradiol drug of combinations mouse |
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