半乳甘露聚糖在侵袭性肺曲霉感染大鼠模型诊断中的意义

目的观察半乳甘露聚糖（GM）在大鼠侵袭性肺曲霉菌感染（IPA）模型不同体液标本不同时间点检测的意义。方法建立大鼠IPA模型,收集不同时间的血浆、支气管肺泡灌洗液（BALF）和尿液标本,检测GM含量,同时进行肺组织病理检查和真菌学培养。结果实验组感染第1、3、5天血浆GM实验平均敏感性分别为58.30%、66.67%、83.33%,BALF分别为66.67%、91.67%、100%,与对照组比较均有统计学差异。所有72只实验大鼠的血浆与BALF标本GM敏感性分别为69.44%、86.11%,特异性分别为100%、100%,阳性预测值分别为100%、100%,阴性预测值分别为68.57%、82.76%。尿液GM检测共5例阳性,敏感性13.89%,特异性100%。血浆及BALF检测GM值随感染天数增加呈升高趋势。结论BALF标本GM检测与血浆标本GM检测具有同样意义,均有助于IPA的早期诊断。

Significance of galactaomannan detection in three kinds of fluid samples from rat model of invasive pulmonary aspergillosis

Objective To assess the value of galactaomannan(GM) detection in three kinds of fluid samples from rat model of invasive pulmonary aspergillosis(IPA).Methods Collected the samples of bronchoalveolar lavage fluid(BALF),plasma and urine for GM detection after the IPA model was established.At the same time,the lung tissue was collected for pathological examination and mycology culture.Results After early infection for 1,3 and 5 days,the average sensitivity of GM test of plasma in experimental group were 58.30%...