| 赤芍总苷联合卡铂对黑色素瘤细胞生长的抑制及相关机制研究 |
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| 引用本文： | 王亚珍,吕品田,王凤红,郑振茹. 赤芍总苷联合卡铂对黑色素瘤细胞生长的抑制及相关机制研究[J]. 医学动物防制, 2011, 0(12): 1112-1114 |
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| 作者姓名： | 王亚珍 吕品田 王凤红 郑振茹 |
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| 作者单位： | 河北省人民医院; |
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| 基金项目： | 河北省科技厅基金资助项目(10276105D-65) |
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| 摘 要： | 目的研究赤芍总苷(total paeonyglucosides,TPG)联合化疗药物卡铂(CBP)对人黑色素瘤A375细胞生长的抑制作用及相关机制。方法用TPG和CBP作用于体外培养的黑色素瘤细胞,SRB法检测药物对黑色素瘤细胞生长的作用,逆转录-聚合酶链反应法(RT-PCR)检测各组细胞PCNA、cyclin D、P21 mRNA的表达情况;Western blot法检测各组细胞PCNA、cyclin D、P21蛋白表达水平。结果 CBP处理组OD值较对照组明显降低,TPG联合CBP导致OD值进一步下降(P<0.01);CBP处理组黑色素瘤细胞PCNA和cyclin D mRNA和蛋白水平均较对照组显著降低(P<0.01),而P21 mRNA和蛋白水平则明显升高(P<0.01);TPG联合CBP作用于黑色素瘤细胞,PCNA和cyclin D mRNA和蛋白水平较CBP单独作用组下降更为明显;P21 mRNA和蛋白水平较CBP单独作用组升高更加显著。结论 TPG和CBP联合用药效果优于CBP单独用药,可进一步提高药物对肿瘤细胞的生长抑制,与降低PCNA和cyclin D表达、升高P21表达有关。
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| 关 键 词： | 赤芍总苷 黑色素瘤细胞 卡铂 化疗药敏性 |
The effect and mechanism of combination therapy with total paeonyglucosides and carboplatin in human malignant melanoma cell |
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| WANG Ya-zhen,LV Pin-tian,WANG Feng-hong,et al.. The effect and mechanism of combination therapy with total paeonyglucosides and carboplatin in human malignant melanoma cell[J]. Chinese Journal of Pest Control, 2011, 0(12): 1112-1114 |
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| Authors: | WANG Ya-zhen LV Pin-tian WANG Feng-hong et al. |
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| Affiliation: | WANG Ya-zhen,LV Pin-tian,WANG Feng-hong,et al. Health Department of Hebei General Hospital,Hebei 050051,China |
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| Abstract: | Objective To study the inhibition effect and mechanism of combination therapy with total paeonyglucosides(TPG) and carboplatin(CBP) in human malignant melanoma A375 cell.Method The human malignant melanoma cells culture in vitro were treated by TPG and CBP in different groups,cell growth was measured by SRB,the mRNA expressions status of PCNA,cyclin D,P21 were determined by RT-PCR and Western blot determined the its protein expression.Results The OD value of CBP treatment group obviously decrease,TPG combin... |
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| Keywords: | Total paeonyglucosides Human malignant melanoma cell Carboplatin Chemosensitivity |
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