| 兔网织红细胞5srRNA对小鼠骨髓瘤细胞SP2/0DNA合成的影响 |
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| 引用本文： | 徐炎,胡雪,张佩瑄,薛社普. 兔网织红细胞5srRNA对小鼠骨髓瘤细胞SP2/0DNA合成的影响[J]. 解剖学报, 1992, 0(2) |
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| 作者姓名： | 徐炎 胡雪 张佩瑄 薛社普 |
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| 作者单位： | 中国医学科学院基础医学研究所细胞生物学研究室,中国医学科学院基础医学研究所细胞生物学研究室,中国原子能科学研究院,中国医学科学院基础医学研究所细胞生物学研究室 北京 100005,北京 100005,北京 100005 |
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| 基金项目： | 国家科委协调攻关局基金 |
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| 摘 要： | 本文报道以~(125)Ⅰ-兔网织红细胞5srRNA温育小鼠骨髓瘤细胞SP2/0后的放射自显影实验,及对宿主细胞DNA合成的影响。结果表明,进入小鼠骨髓瘤细胞中的兔网织红细胞5srRNA集中在核内,~3H-TdR参入实验表明,兔网织红细胞5srRNA进入SP2/O核内后,显著抑制了核内DNA合成和肿瘤细胞的分裂活动。以上结果对哺乳类网织红细胞胞质中存在调控肿瘤细胞生长的负调节因子提供了实验依据。
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| 关 键 词： | 网织红细胞5srRNA DNA合成 放射自显影 ~3H-胸腺嘧啶核苷 |
EFFECT OF RABBIT RETICULOCYTES 5srRNA ON DNA SYNTHESIS OF MOUSE MYELOMA CELLS SP2/0 |
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| Xu Yan,Hu Xue,Zhang Peixuan,Xue Shepu. EFFECT OF RABBIT RETICULOCYTES 5srRNA ON DNA SYNTHESIS OF MOUSE MYELOMA CELLS SP2/0[J]. Acta Anatomica Sinica, 1992, 0(2) |
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| Authors: | Xu Yan Hu Xue Zhang Peixuan Xue Shepu |
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| Abstract: | 5srRNA was isolated and purified from the reticulocytes of rabbit. It labeled with ~(125)I, then incubated with mouse myeloma cells (SP2/0). By autoradiography it was observed that ~(125)I-SsrRNA could pass into the nuclei of the cells. In a separate experiment, it showed that the incorporation rate of ~3H-TdR into nuclei of SP2/0 after incubation with 5srRNA was decreased as compared with that f control group, hence the result indicates that 5srRNA inhibits DNA synthesis of the SP2/0 cells and it seems to play a role in the regulation of gene expression through its hybridization with DNA sequences of the SP2/0 cells. Thus it is likely that 5srRNA might act as "erythroid denucleation factor". |
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| Keywords: | Reticulocytes 5srRNA DNA synthesis Autoradiography ~3H-TdR |
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