雷公藤甲素脂质体制备及体内抗肿瘤实验研究

目的注入法制备雷公藤甲素脂质体,观察其形态特征和包封率,并对其体内抗H22疗效进行初步研究。方法用注入法制备雷公藤甲素脂质体,通过普通光学显微镜和动态光散色粒度分析仪测定其形态、粒径,用HPLC测定其包封率;并建立H22荷瘤模型观察其抗肿瘤活性。结果该法所得脂质体形态规则、粒度均匀,平均直径约为45.6 nm,但包封率不是很高约为46.7%;体内抗肿瘤实验结果表明,雷公藤甲素脂质体具有较强的抗H22活性,且与浓度呈正相关,而且相对"游离"雷公藤甲素毒性较小。结论雷公藤甲素脂质体具有较强的抗肿瘤活性,且不良反应较小。

Preparation of Triptolide-liposome and Its in vivo Antitumor Activity

OBJECTIVE To prepare triptolide-liposome by infusion method,and investigte the properties,such as morphology,encapsulation efficiency,and anti-H22 cell lines effect in vivo.METHODS Triptolide-liposome was obtained by infusion method,its morphologic characteristics and encapsulation efficiency was detected by optical microscope,dynamic light scattering instrument and HPLC,respectively.Otherwise,its anti-H22 cell lines effect was evaluted by using mice bearing H22 tumor. RESULTS The shape of triptolide-liposome was regular and narrowly-distributed with average diameter of 45.6nm;but its encapsulated efficiency was just reached 46.7%;In vivo anti-tumor assay indicated that triptolideliposome had strong anti-H22 tumor activity,which was positively correlated with its concentration,and manifested less toxicity than free triptolide only.CONCLUSION Triptolide-liposome has great anti-tumor activity with less toxicity.