| PUVA诱导K562细胞凋亡时对Fas表达的影响 |
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| 引用本文： | 张励,陈楠楠,刘小宇,王伟,黄世林.PUVA诱导K562细胞凋亡时对Fas表达的影响[J].临床军医杂志,2008,36(2):163-165. |
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| 作者姓名： | 张励 陈楠楠 刘小宇 王伟 黄世林 |
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| 作者单位： | 解放军第210医院,中医血液科,辽宁,大连,116021 |
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| 摘 要： | 目的探讨补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞K562凋亡时对Fas表达的影响。方法以K562细胞为研究对象,以细胞凋亡率、电镜下细胞超微结构改变以及细胞Fas基因表达水平、蛋白表达水平为检测指标,观察中药补骨脂中提取的PSO加波长为360 nm的UVA对人白血病细胞诱导凋亡的作用以及部分作用途径,并采用多因素方差分析法进行统计学处理。结果(1)单纯PSO、单纯UVA照射及PUVA均可诱导K562细胞发生凋亡,PUVA的作用显著强于前两者。(2)电镜下观察经PUVA处理后的K562细胞超微结构,可见明显的凋亡形态学改变。(3)单纯PSO、单纯UVA照射及PUVA均可上调K562细胞Fas基因表达水平,PUVA的作用显著强于前两者。(4)单纯PSO、单纯UVA照射及PUVA均可上调K562细胞Fas蛋白表达水平,PUVA的作用显著强于前两者。结论(1)PUVA可诱导白血病细胞K562发生凋亡,作用强于单纯PSO及单纯UVA照射。(2)PUVA诱导K562凋亡的途径之一为上调K562细胞Fas基因表达水平。
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| 关 键 词： | 补骨脂素 长波紫外线 白血病 K562细胞 凋亡 Fas |
Effect of PUVA on Apoptosis and Expression of Fas in K562 Leukemia Cells |
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| Zhang Li,Chen Nan-nan,Liu Xiao-yu,Wang Wei,Huang Shi-lin.Effect of PUVA on Apoptosis and Expression of Fas in K562 Leukemia Cells[J].Clinical Journal of Medical Officer,2008,36(2):163-165. |
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| Authors: | Zhang Li Chen Nan-nan Liu Xiao-yu Wang Wei Huang Shi-lin |
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| Institution: | Zhang Li,Chen Nan-nan,Liu Xiao-yu,Wang Wei,Huang Shi-lin(Department of Traditional Chinese Medicine , Hematology,PLA No.210 Hospital,Dalian 116021,China) |
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| Abstract: | Objective To explore the effect of psoralen(PSO) and long-wave ultraviolet A(UVA) on apoptosis and expression of Fas in K562 leukemia cells.Methods K562 cells were taken as the study objects and their apoptosis ratios,ultrastructuse changes and expression of Fas were detected in order that the effect of PSO extracted from Chinese herbal medicine and UVA(360 nm) on human leukemia cells could be observed.The factorial design and analysis of variance were used to analyze the interaction among the factors.Resul... |
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| Keywords: | Fas |
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