孕期酒精暴露对子鼠海马神经元凋亡的影响

目的:研究孕期酒精暴露对子鼠海马神经元凋亡的影响.方法:采用健康C57BL/6J小鼠,用胃饲酒精法建立孕期酒精暴露模型.90只孕鼠随机分为3组: 对照组、低剂量酒精组和高剂量酒精组.低、高剂量酒精组分别按2.0 g/kg与4.0 g/kg的剂量给予体积分数为25%的乙醇,1次/d,直至子鼠出生,对照组自由饮食.3组取新生鼠、生后7 d与生后14 d的小鼠各10只,取海马组织进行caspase-3免疫组化染色,以观察海马神经元的凋亡.结果:低剂量酒精组和高剂量酒精组各年龄子鼠海马神经元caspase-3阳性率明显高于对照组(P＜0.01);高剂量酒精组海马神经元caspase-3阳性率明显高于低剂量酒精组(P＜0.01).结论:孕期酒精暴露可激活发育期海马神经元caspase-3,诱发神经元凋亡,可能与出生子鼠智力障碍密切相关.

Effects of alcohol exposure during pregnancy on neuroapoptosis of hippocampus in filial mice

Aim:To investigate the effects of alcohol exposure during pregnancy on neuroapoptosis of hippocampus in filial mice.Methods:Clean-grade C57BL/6J mice were used.The pregnant mice were randomly divided into 3 groups:control group,low-dose alcohol group and high-dose alcohol group.Mice in low or high-dose alcohol groups received 25% ethanol by gavage at a dose of 2.0 g/kg and 4.0 g/kg,respectively,once per day,till the pup's birth,and the control group were allowed free access to food and water.Caspase-3 staining was performed in hippocampus in neonatal,7-day old and 14-day old mice from the 3 groups to detect neuronal apoptosis.Results:The caspase-3 positive rate in low-dose alcohol group and high-dose alcohol group was obviously higher than that in control group(P<0.01),and the caspase-3 positive rate in high-dose alcohol group was higher than that in low-dose alcohol group(P<0.01).Conclusion:The prenatal alcohol exposure can activate caspase-3 and induce neuronal apoptosis in the developing hippocampus of mice,which may be closely related to low intelligence of filial mice.