端粒酶活性与细胞凋亡在尖锐湿疣患者组织中的表达

目的 探讨尖锐湿疣(CA)皮损中端粒酶活性与细胞凋亡表达情况及其两者之间的相关性。方法 采用端粒重复序列扩增文件-酶标法(TRAP-ELISA)检测了30例尖锐湿疣患者皮损端粒酶活性,并与正常组织和肿瘤细胞株作对照;原位末端标记法(TUNEL)检测皮损中细胞凋亡。结果 27例(90%)尖锐湿疣皮损中端粒酶活性A₄₅₀值为0.50~2.76,平均1.302,明显高于正常皮肤组织,差异有显著性(t=6.12,P<0.01);24例皮损中有凋亡细胞,占80%,且端粒酶活性与凋亡指数呈显著负相关,r=-0.52,P=0.004。结论 端粒酶可以在一些非恶性皮肤病中表达,且影响细胞凋亡,可能参与CA发病。

Telomerase Activity in Condyloma Acuminatum: Correlation with Decreased Apoptosis

Objective To study the telomerase activity,the keratinocyte apoptosis in condyloma ac-cuminatum(CA)and the correlation between them.Methods CA specimens from30patients were stud-ied,and compared with normal tissue and tumor cell lines.Telomerase activity was detected with telomeric repeat amplification polymerase chain reaction(TRAP)-ELISA.Terminal deoxynucleotidyl transferase(TdT)-mediated biotin dUTP nick end-labeling(TUNEL)was used to evaluate apoptotic cells.Results Increased telomerase activity was detected in27(90%)patients with CA,with A 450 ranging between0.50and2.76(mean1.3022).Apoptotic keratinocytes were found in24out of30CA cases(80%).A statistically signifi-cant inverse correlation was found between telomerase activity and apoptotic index(r =-0.52,P=0.004).Conclusion Keratinocyte telomerase is activated in condyloma acuminata,which is correlated with the downregulation of apoptosis,thus they might be involved in the pathogenesis of CA.