Expression and Bioinformatics Analysis of SPACA4 in Human and Mice |
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Authors: | Ai-fa TANG Zhen-dong YU Yao-ting GUI Xin GUO Xian-xin LI Wei-xiang LIU Hui ZHU Zhi-ming CAI |
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Institution: | 1. Shenzhen Key Lab of Male Reproduction and Genetics, Shenzhen Hospital of Peking University, Shenzhen 518036, China 2. Research Center of Biomedical Engineering, Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China |
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Abstract: | Objective To analyze the expression of SPACA4 in human and mice. Methods Testes cRNA samples from Balb/c mice of different postnatal days were performed with mouse affymetrix chip to screen the expression of SPACA4 in mice. Sub-quantitative RT-PCR and bioinformatic tools were used here to describe the expression profile of SPACA4 in mice and human. Results The results of gene chip analysis indicated that the expression of mSPACA4 began after d 35 of postnatal testis in mice. Sub-quantitative RT-PCR assay showed that SPACA4 gene expressed exclusively in mouse and human testis, and mouse mSPACA4 gene expressed after d 35 of postnatal testis that was consistency with the results of gene chip analysis. By bioinformatics analysis, mSPACA4 is located in cell membrane (34.8%) or plasma membrane (34.8%), the signal peptide cleavage site between position 19 and 20 amino acids, transmembrane region between 2-20 and 101-126 amino acids, respectively, on mSPACA4 protein. Conclusion mSPACA4 and hSPACA4 were testis-specific genes, and the expression of mSPACA4 begins after d 35 of postnatal testis in mice. SPACA4 is a candidate for targeting in a sperm-based contraceptive vaccine. |
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Keywords: | gene chips SPACA4 gene spermatogenesis male contraceptive vaccine |
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