Teniposide-induced changes in the physical properties of phosphatidylcholine liposomes. A calorimetric study |
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| Authors: | S E Wright J C White |
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| Affiliation: | 1. Department of Biochemistry, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27103, U.S.A.;1. Department of Physics, Panimalar Engg. College, Chennai 600123, Tamilnadu, India;2. Department of Physics, Presidency College, Chennai 600005, Tamilnadu, India;3. Department of Physics, Arignar Anna Government Arts College, Cheyyar 604407, Tamil Nadu, India;4. Department of Physics, Madras Christian College, East Tambaram 600059, Tamil Nadu, India;5. Department of Mathematics, Sri Chandrasekharendra Saraswathi Viswa Maha Vidyalaya, Kanchipuram 631561, India;1. Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China;2. School of Environment & Natural Resources, Renmin University of China, Beijing 100872, PR China;3. Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China;1. Department of Pharmaceutical Sciences, Medical College, Henan University of Science & Technology, Luoyang 471003, PR China;2. Department of Marketing, Henan University of Animal Husbandry and Economy, Zhengzhou 450045, PR China;1. Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, Oregon, USA;2. Department of Medicine, Knight Cardiovascular Institute, Oregon Health and Science University, Portland, Oregon, USA;3. Department of Cell, Developmental and Cancer Biology, Oregon Health and Science University, Portland, Oregon, USA;4. Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA;1. Department of Chemistry, Kuwait University, Faculty of Science, P.O. Box 5969, 13060 Safat, Kuwait;2. Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, NY 12222, USA |
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| Abstract: | The anticancer agent teniposide has significant effects on plasma membrane components, in addition to its well known nuclear effects. Alterations in the properties and function of cellular membranes by various amphipathic compounds have been attributed previously to their relatively non-specific interactions with membrane components. We have examined the interaction of teniposide with defined model membranes by monitoring drug-induced changes in the melting profile of phospholipids by differential scanning calorimetry. The main phase transition temperature of dimyristoyl- or dipalmitoylphosphatidylcholine was lowered and broadened by the presence of teniposide in the liposomes. These effects were essentially linear over the concentration range of 1-5 mole %. The calorimetric enthalpy of the gel to liquid-crystalline transition of the phospholipids was not changed by the addition of the drug. The characteristic pretransition of these saturated phospholipids was decreased by teniposide concentrations as low as 0.1 mole % and was abolished at teniposide concentrations greater than 1 mole %. The data confirm the lipophilic nature of teniposide and indicate that the non-specific interactions with membrane lipids should be considered when evaluating the membrane-related effects of this agent. |
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