2型糖尿病患者龈沟液中基质金属蛋白酶-1的检测和临床意义 |
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引用本文: | 丁岩,林杰,凌厉,卜瑞芳. 2型糖尿病患者龈沟液中基质金属蛋白酶-1的检测和临床意义[J]. 中华糖尿病杂志, 2014, 22(11): 1012-1014 |
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作者姓名: | 丁岩 林杰 凌厉 卜瑞芳 |
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作者单位: | 1. 江苏省无锡市精神卫生中心口腔科,无锡,214000 2. 解放军第101医院检验科 3. 无锡市人民医院内分泌科 |
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基金项目: | 南京医科大学科技发展基金面上项目 |
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摘 要: | 目的 比较T2DM患者、T2DM合并牙周病患者与正常对照者龈沟液(GCF)中基质金属蛋白酶-1(MMP-1)和GCF量,以及T2DM患者GCF中MMP-1量与糖代谢状况的关系. 方法 使用滤纸条法采集GCF.选取T2DM组、T2DM合并牙周病组各30例,正常对照组30名,ELISA测定GCF中MMP-1含量,运用高效液相色谱法检测HbA1c水平. 结果 T2DM、T2DM合并牙周病组GCF量高于正常对照组[(2.81±1.58)、(3.56±2.96)vs(1.02±0.65) μl/样本](P<0.01).T2DM合并牙周病组GCF中MMP-1量高于T2DM、正常对照组[(5.02±0.16)vs(3.01土0.09)、(2.24±0.11) μl/样本](P<0.01).T2DM组与正常对照组GCF中MMP-1量比较差异无统计学意义(P>0.05).T2DM合并牙周病组GCF中MMP-1量与HbA1c水平无相关性(r=0.153,P=0.151). 结论 3组GCF及MMP-1水平在T2DM合并牙周病组中最高,在对照组最低,提示牙周炎症是影响结果的主要原因.MMP-1水平在T2DM组中低于T2DM合并牙周病组,提示T2DM患者不只是MMP-1水平改变而导致牙周病的易感,可能是多个因素共同作用的结果.
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关 键 词: | 糖尿病,2型 牙周病 龈沟液 基质金属蛋白酶-1 |
Measurement of MMP-1 in gingival crevicular fluid and its clinical significance in patients with type 2 diabetes mellitus |
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Affiliation: | D ING Yan ,LIN J ie ,LING Li ,et al. (Department of Stomatology , Wuxi Mental Health Center Affiliated to NJMU, Wuxi 214000, China) |
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Abstract: | Objective To investigate the level of MMP-1 in gingival crevicular fluid and its clinical significance in patients with type 2 diabetes mellitus and the relationship between MMP-1 concentration and glycometabolism in T2DM patients.Methods GCF collection was performed with the filter paper method.Patients with T2DM (n=30) and T2DM plus periodontal diseases (n=30),and healthy controls (n=30) were selected in this study.The MMP-1 levels in GCF were measured by ELISA,and HbA1c levels were measured by high-performance liquid chromatography.Results The GCF volume was higher in diabetic group and T2DM plus periodontal diseases group than in normal control group [(2.81± 1.58),(3.56 ± 2.96) vs (1.02 ± 0.65) μl/sample] (P 〈 0.01) and higher in T2DM plus periodontal diseases than in diabetes alone and normal control [(5.02 ± 0.16) vs (3.01 ± 0.09),(2.24 ± 0.11) μl/sample] (P〈0.01).There was no statistically significant difference in MMP-1 level of GCF between diabetes alone and normal control (P=0.151).There was no correlation of the MMP-1 level in GCF with HbA1c levels in group of T2DM plus periodontal diseases.Conclusion Among three groups,GCF weight and its MMP-1 levels are the highest in group of T2DM group plus periodontal diseases and the lowest in normal control group,which suggests that periodontitis is the main reason affecting the results.MMP-1 level is lower in diabetic group than that in T2DM plus periodontal diseases group,which suggests that the periodontal disease in diabetics is not only caused by changes of MMP-1 level,but also by several other factors. |
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Keywords: | Diabetes mellitus,type 2 Periodontal disease Gingival crevicular fluid(GCF) Matrix metalloproteinases-1 (MMP-1) |
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