|
|||||
|
|
| 碱烧伤后角膜新生淋巴管的实验研究 | |
| 引用本文： | 凌士奇,柳灿,黎韦华,林浩添.碱烧伤后角膜新生淋巴管的实验研究[J].眼科新进展,2010,30(5). |
| 作者姓名： | 凌士奇 柳灿 黎韦华 林浩添 |
| 作者单位： | 1. 广州市妇女儿童医疗中心,广东省广州市,510120 2. 广州市白云区红十字会医院眼科,广东省广州市,510545 3. 中山大学中山眼科中心,广东省广州市,510060 |
| 基金项目： | 国家自然科学基金资助(编号:30700927); 广东省科技计划项目基金资助(编号:93068)~~ |
| 摘 要： | 目的动态检测大鼠角膜碱烧伤后的角膜新生淋巴管和血管的变化,并阐明二者之间的关联。方法制备大鼠角膜碱烧伤模型,于碱烧伤后1周、2周行角膜组织电镜检查,检测角膜新生淋巴管与新生血管。5’核苷酸酶-碱性磷酸酶(5’-nase-alkaline phos-phatase,5’-NA-ALP)双重酶组织化学染色及全角膜免疫荧光染色分别检测碱烧伤后6h、1d、3d、1周、2周、3周、4周、5周、6周、7周、8周的角膜新生淋巴管和新生血管的动态变化,并进行淋巴管计数(lymphatic vessels counting,LVC)和血管计数(blood vessels counting,BVC)比较。结果电镜观察结果:角膜碱烧伤后1周,角膜基质层出现新生血管,未见淋巴管;碱烧伤后2周,新生血管和新生淋巴管均出现。酶组织化学染色结果显示:碱烧伤后6h有新生血管,1周时角膜基质层存在新生淋巴管,2周时LVC和BVC均达到高峰,分别为(16.41±1.00)个和(50.40±1.56)个;以后逐渐下降,5周时LVC为(0.33±0.50)个,BVC为(12.52±2.51)个;8周时均为0。碱烧伤后,新生淋巴管和新生血管呈显著性正... |
| 关 键 词： | 角膜 碱烧伤 新生淋巴管 |
Experimental study on corneal new lymphatic vessels after alkali burn |
|
| LING Shi-Qi,LIU Can,LI Wei-Hua,LIN Hao-Tian From the Guangzhou Women , Children\'s Medical Center,Guangzhou ,Guangdong Province,China.Experimental study on corneal new lymphatic vessels after alkali burn[J].Recent Advances in Ophthalmology,2010,30(5). | |
| Authors: | LING Shi-Qi LIU Can LI Wei-Hua LIN Hao-Tian From the Guangzhou Women Children\'s Medical Center Guangzhou Guangdong Province China |
| Institution: | LING Shi-Qi,LIU Can,LI Wei-Hua,LIN Hao-Tian From the Guangzhou Women , Children\'s Medical Center,Guangzhou 510120,Guangdong Province,China,Department of Ophthalmology,Guangzhou Baiyun District Red Cross Hospital,Guangzhou 510545,Zhongshan Ophthalmologic Center,Sun Yat-Sen University,Guangzhou 510060 |
| Abstract: | Objective To study the changes of corneal new lymphatic vessels and blood vessels after corneal alkali burn in rat,and discuss their relationship.Methods The models of corneal alkali burn in rat were established,the new lymphatic vessels and blood vessels in corneal tissues were examined by electron microscope at 1 week and 2 weeks after alkali burn.The dynamic changes of corneal new lymphatic vessels and blood vessels were detected by 5'-nase-alkaline phosphatase double-enzyme-histochemistry staining and w... |
| Keywords: | cornea alkali burn new lymphatic vessel |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |