| HPLC同时测定川芎-天麻药对中4种指标性成分的含量 |
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| 引用本文： | 周赛妮,刘明平,文艺,严萍,毛禹康,黄志云. HPLC同时测定川芎-天麻药对中4种指标性成分的含量[J]. 中国实验方剂学杂志, 2016, 22(3): 53-56 |
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| 作者姓名： | 周赛妮 刘明平 文艺 严萍 毛禹康 黄志云 |
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| 作者单位： | 广州中医药大学中药学院, 广州 510006,广州中医药大学中药学院, 广州 510006,广州中医药大学第一附属医院, 广州 510405,广州中医药大学中药资源研究与工程中心, 中药资源科学教育部重点实验室, 广州 510006,广州中医药大学中药学院, 广州 510006,广州中医药大学中药学院, 广州 510006 |
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| 基金项目： | 国家自然科学基金项目(81274107) |
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| 摘 要： | 目的:建立川芎-天麻药对中天麻素、天麻苷元、阿魏酸和藁本内酯4种成分的含量测定方法。方法:采用Inertsil ODS-3色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-0.05%磷酸水梯度洗脱,检测波长220 nm(天麻素、天麻苷元),320 nm(阿魏酸、藁本内酯),柱温25℃,流速1.0 mL·min-1。结果:4种效应成分分离度良好,标准曲线在检测范围内线性关系良好,天麻素、天麻苷元、阿魏酸和藁本内酯的平均回收率分别为104.0%,103.4%,100.6%,101.8%,RSD分别为0.4%,0.5%,4.5%,2.6%。结论:该方法简便、精密度、重复性好,可用于川芎-天麻药对中4种效应成分的含量测定。
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| 关 键 词： | 天麻素 天麻苷元 阿魏酸 藁本内酯 川芎-天麻药对 |
| 收稿时间： | 2015-01-29 |
Simultaneous Determination of Four Index Components in Extracts of Ligusticumc chuanxiong-Gastrodia elata by HPLC |
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| ZHOU Sai-ni,LIU Ming-ping,WEN Yi,YAN Ping,MAO Yu-kang and HUANG Zhi-yun. Simultaneous Determination of Four Index Components in Extracts of Ligusticumc chuanxiong-Gastrodia elata by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2016, 22(3): 53-56 |
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| Authors: | ZHOU Sai-ni LIU Ming-ping WEN Yi YAN Ping MAO Yu-kang HUANG Zhi-yun |
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| Affiliation: | School of Traditional Chinese Medicine(TCM), Guangzhou University of Chinese Medicine, Guangzhou 510006, China,School of Traditional Chinese Medicine(TCM), Guangzhou University of Chinese Medicine, Guangzhou 510006, China,First Affiliated Hospital of TCM, Guangzhou 510405, China,Ministry of Education Key Laboratory for Chinese Herbal Resource Science, Research Centre of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China,School of Traditional Chinese Medicine(TCM), Guangzhou University of Chinese Medicine, Guangzhou 510006, China and School of Traditional Chinese Medicine(TCM), Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | gastrodin 4-hydroxybenzyi aicohol ferulic acid ligustilide Ligusticumc chuanxiong-Gastrodia elata |
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