河南产大黄类药材质量分析

目的:比较河南不同产地大黄的质量,为该药材的利用与开发提供参考。方法:检查土大黄苷,利用薄层色谱进行定性鉴别,采用HPLC测定游离蒽醌类成分的含量,流动相甲醇-0.1%磷酸溶液(85:15),检测波长254 nm。结果:9批药材中4批含有土大黄苷,鉴别为土大黄,来源为波叶组大黄的华北大黄;5批不含土大黄苷,大黄游离蒽醌类成分质量分数均>1.5%,符合《中国药典》2010年版的规定,平顶山鲁山县的大黄中游离蒽醌类成分的含量较高(4.65%),洛阳嵩县次之(4.38%),2种大黄来源分别为药用大黄、掌叶大黄。结论:河南不同产地分布的药用大黄、掌叶大黄药材符合《中国药典》2010年版的规定,具有开发利用前景;华北大黄不能作为大黄药材应用于临床。

Quality Analysis of Rhei Radix et Rhizoma in Henan

Objective: To provide basis for utilization and development of Rhei Radix et Rhizoma through comparing its quality from different areas in Henan. Method: Rhapontin was checked,qualitative identification was determined by TLC,HPLC was employed to determine the content of free anthraquinones with mobile phase of methanol -0.1% phosphoric acid (85:15) and detection wavelength at 254 nm. Result: In 9 batches of samples,4 batches of Rhei Radix et Rhizoma contained rhapontin,it was identified as Rheum franzenbachii;5 batches did not contain rhapontin,the content of free anthraquinones all exceed 1.5% and conformed to rules in the 2010 edition of Chinese Pharmacopoeia,the content of free anthraquinones (4.65%) was higher in samples from Lushan county,followed by samples from Song county with 4.38%,These two kinds of samples were from R.officinale and R. palmatum,respectively. Conclusion: R. officinale and R. palmatum from different areas in Henan are all consistent with rules in the 2010 edition of Chinese Pharmacopoeia,it is worthy of exploitation and utilization.R. franzenbachii cannot be applied in medicinal materials as Rhei Radix et Rhizoma.