胚胎干细胞试验预测胚胎毒性替代方法进展

小鼠胚胎干细胞试验(EST)是通过ECVAM正式验证的胚胎和发育毒性的动物试验替代方法。在新技术的推动下,多项优化的EST方法通过基因转染、利用人来源的干细胞、增加检测通量、组合检测终点、量化检测指标等方式,提高了EST方法预测胚胎毒性的科学相关性和检测效率。在整合测试策略的原则之下,经过大规模化学物质的前验证和标准化试验,新的EST方法可单独或作为组合试验的一部分可以应用于先导药物的研发和化学品的胚胎毒性预测及机制研究。

Progress for Alternatives Methods Predict the Embrytoxicity by Embryonic Stem cell Test

Embryonic Stem cell Test(EST) is an alternative testing method for testing embryotoxicity and developmental toxicity which have been validated by the European Centre for Validation of Alternative Methods(ECVAM).Under the promote of new technology, EST method has been refined by many ways including gene transfection, utilizing human-derived stem cell, increase the assay throughput, integrating test endpoints and quantifying the test indexs,which enhanced scientific relevance and testing efficiency.Within the principle of integration testing strategy(ITS),conducting pre-validation tests and standardized experiments on a large scale of chemicals, the new EST method as a individual test or a part of integrated test systerm is promising to apply on the research of lead drugs and the embryotoxicity prediction and mechanism study of chemicals.