大鼠脑缺血再灌注后梗死体积的动态变化

目的观察大鼠大脑中动脉缺血再灌注后梗死灶体积的变化规律。方法线栓法制作大鼠局灶性脑缺血再灌注模型,观察脑缺血2h再灌注3h、24h、3d、7d、14d及21d后的神经功能缺损评分及2％氯化三苯基四氮唑(TTC)标记的梗死体积。结果缺血2h再灌注3h组已经出现较明显的梗死灶(梗死体积占前脑体积14．4％),再灌注24h组梗死体积最大(24．3％),显著大于再灌注3h、7d、14d、21d组(P<0．05)。再灌注3d组梗死灶仍较大 (23．8％),再灌注7d组梗死体积缩小(5．0％),再灌注14d组梗死灶进一步缩小(1．2％),再灌注21d组梗死灶基本消失(0．2％)。大鼠神经功能缺损评分与梗死体积之间呈显著相关(r=0．61,P<0．01)。结论脑缺血再灌注后梗死体积于24h达最大,21d时基本消失。脑缺血再灌注后神经功能缺损评分与梗死体积之间显著相关。

Dynamic change of infarction volume in reversible middle cerebral artery occlusion rats

Objective To investigate the dynamic change of infarction volume in reversible middle cerebral artery occlusion rats. Methods The focal brain ischemia-reperfusion model was induced by inserting a suture through the internal carotid artery in rats. The infarction area was marked by 2% triphenyltetrazolium chloride (TTC) at 3h,24h,3d,7d,14d and 21d after brain ischemia-reperfusion. The scores of neurological deficits were determined at the above mentioned time points. Results The infarction was detected at 3h after ischemia-reperfusion (infarction volume/forebrain volume = 14. 4%). The infarction volume peaked at 24h after ischemia-reperfusion (24. 3% ) which is significantly larger than that at 3h ,7d, 14d ,21d(eachP<0. 05). The infarction volume remained large at 3d (23. 8%) .decreased at 7d (5. 0%) and almost disappeared at 21d(0. 2%) after ischemia-reperfusion. The infarction volume correlated significantly with the score of neurological deficits (r = 0. 61,P<0. 01). Conclusions The infarction volume peaked at 24h and almost disappeared at 21d after brain ischemia-reperfusion in rats,which correlated significantly with the score of neurological deficits.