人参多糖体外诱导人非小细胞肺癌A549细胞凋亡的实验研究

目的研究人参多糖体外对人非小细胞肺癌A549细胞增殖及凋亡的作用。方法应用MTT法、流式细胞仪、Annexin V-PI双标、Hoechest荧光染色、TUNEL法等多种方法,体外研究人参多糖对A549细胞的凋亡作用；采用免疫组化的方法,观察药物作用后bcl-2表达的变化。结果人参多糖对A549细胞生长具有抑制作用,并有促凋亡效应；对细胞周期的影响表现为G_0/G_1期的阻滞,未见bcl-2表达的下调。电镜和Hoechst、TUNEL法都可观察到凋亡形态学的改变。结论人参多糖可抑制人非小细胞肺癌A549细胞的生长,并诱导细胞发生凋亡,这有可能是人参多糖抗肿瘤作用机制之一,其凋亡的发生与bcl-2的表达无明显关系。

Experimental Study of the A549 Cell Apoptosis of Human Non-small Cell Lung Cancer Induced by Ginseng Polysaccharide in vitro

Objective To study the in vitro effect of Ginseng polysaccharide on the proliferation and apoptosis of human non-small cell lung cancer.Methods The apoptosis of A549 cell induced by Ginseng polysaccharide was detected by MTF method,flow cytometry,Annexin V-PI double-labeling,Hoechest luorescent staining and TUNEL.The change of bcl-2 expression after Ginseng polysaccharide treatment was observed by immunohistochemi- cal method.Results Ginseng polysaccharide had inhibitive action on A549 cell growth,and apoptosis promoting effect as well.Its effect on cell cycle was manifested as G_0/G_1,phase arrest.No down-regulation of bcl-2 expression was seen.Morphological changes of apoptosis could be observed under electron microscope,Hoechst and TUNEL techniques.Conclusion Ginseng polysaccharide could inhibit the growth of human non-small cell lung cancer A549 cell,and induce the cell apoptosis,which might be one of the anti-cancer mechanisms of Ginseng polysaccharide. The occurrence of cell apoptosis had no clear relationship with the expression of bcl-2.