温病湿热证湿热量化的相关性实验研究

目的;为探讨温病湿热证湿热量化的实验方法,在以往研究基础上,筛选出相关性强的量化指标。方法;通过复制湿热证动物模型,采用Bradford方法测定水通道蛋白2(AQP2)含量,放免法检测内皮素(ET)、降钙素基因相关肽(CGRP),一步双抗夹心酶联免疫法检测粒细胞集落刺激因子(G-CSF),比色法检测中分子(MMS)和巯基(R-SH)物质等。结果;湿热证模型组AQP2水平较正常组明显降低,用清香散治疗后AQP2水平基本恢复正常,与正常组接近。湿热模型组ET水平较正常对照组明显升高,而CGRP较正常组降低(P<0.01)。湿热证模型大鼠血中G-CSF、MMS升高,而R-SH水平降低,经清香散治疗1周后.大鼠血中G-CSF、MMS及R-SH基本恢复正常,模型对照组G-CSF阳性率升高。结论;AQP2与ET、CGRP、G-CSF、R-SH、MMS分别可作为“湿”偏重,“热”偏重,“湿热病因”的相关性量化指标。这些技术指标对湿热定性、量化,清热祛湿药物的研究以及湿热证“证”本质的深入探讨有重要意义。

Experimental Study on Correlativity of Damp-Heat Quantification in Damp-Heat Syndrome of Febrile Disease

Objective: To explore experimental methods for damp-heat quantification in damp-heat syndrome of epidemic febrile disease and scan highly correlative quantification indexes. Methods: AQP2. endothenin (ET ). calcitonin-gene related peptide (CGRP) and granulocyte colony stimulating factor (G-CSF) . middle molecular substance (MMS) and hydrosulfide substances (R-SH) were determined in the damp-heat animal model. Results: AQP2 level in damp-heat model group was significantly lower than that in normal control group, and after treatment by Qingxiang Powder it was restored basically to the normal level. ET level in damp-heat model group was higher and CGRP was lower than that in normal group (P<0. 01) . Blood G-CSF and MMS increased and R-SH level decreased in the damp-heat rats. After treatment of one week by Qingxiang Powder, blood G-CSF. MMS and R-SH basically restored to normal, and G-CSF positive rate increased in model control group. Conclusion: AQP2 ET. CGRP. G-CSF. R-SH and MMS can be respectively used as correlative quantification indexes for the patient with "more dampness" . "more heat" and "the cause of disease of damp-heat".