| 眼镜蛇镇痛素najanalgesin对大鼠神经病理性疼痛脊髓GLT-1的影响 |
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| 引用本文： | 林勤剑,江伟健,梁映霞,韩丽萍,张翠兰,赵树进. 眼镜蛇镇痛素najanalgesin对大鼠神经病理性疼痛脊髓GLT-1的影响[J]. 中国中药杂志, 2011, 36(7): 903-907 |
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| 作者姓名： | 林勤剑 江伟健 梁映霞 韩丽萍 张翠兰 赵树进 |
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| 作者单位： | 1. 中山大学,中山医学院,药理学系,广东,广州,510080;广州军区广州总医院,药学部,广东,广州,510010
2. 广州军区广州总医院,药学部,广东,广州,510010 |
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| 基金项目： | 全军医学科学技术研究"十一五"计划项目;军队医药卫生科研基金课题(06MA125) |
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| 摘 要： | 目的:观察眼镜蛇镇痛素najanalgesin对L5脊神经结扎并剪断(spinal nerve ligation and transection,SNL)大鼠脊髓胶质细胞谷氨酸转运体亚型1(GLT-1)表达的影响,探讨najanalgesin脊髓镇痛机制.方法:100只雄性SD大鼠,随机分为假手术组(A),SNL模型组(B),SNL+najanalgesin组(C),SNL+生理盐水对照组(D),SNL+najanalgesin+脂质体组(E),SNL+najanalgesin+脂质体+GLT-1反义寡核苷酸(As-ODNs,F)6组.鞘内分别注射10μL生理盐水(A,D),40ng·kg-1najanalgesin(C,E,F),每日1次,在注射najanalgesin的同时于第3天一次性注射脂质体+GLT-1As-ODNs10μL(F)以及脂质体10μL(E),术后1,4,7 d(A,B,C,D)及第7天(E,F)取各组大鼠L4～L6脊髓节段,检测各组GLT-1 mRNA和蛋白表达改变.结果:采用SNL成功建立了神经病理性疼痛模型.与假手术组相比,B,D组大鼠GLT-1 mRNA和蛋白表达先增加再降低,C组大鼠GLT-1 mRNA和蛋白表达明显增加,但不随时间变化.与D组相比,C组大鼠第7天GLT-1 mRNA和蛋白表达明显增高.与椎管内注射najanalgesin组相比,椎管内注射GLT-1As-ODNs后,F组GLT-1表达明显下降,而脂质体对照组GLT-1的表达基本不变.结论:najanalgesin可以增加脊髓GLT-1 mRNA和蛋白表达,是其脊髓镇痛机制之一.
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| 关 键 词： | 眼镜蛇毒 神经病理性疼痛 胶质细胞谷氨酸转运体1 脊神经结扎 |
| 收稿时间： | 2010-08-24 |
Influence of najanalgesin from Naja naja on GLT-1 in spinal cord of rat in neuropathic pain |
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| LIN Qinjian,JIANG Weijian,LIANG Yingxi,HAN Liping,ZHANG Cuilan and ZHAO Shujin. Influence of najanalgesin from Naja naja on GLT-1 in spinal cord of rat in neuropathic pain[J]. China Journal of Chinese Materia Medica, 2011, 36(7): 903-907 |
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| Authors: | LIN Qinjian JIANG Weijian LIANG Yingxi HAN Liping ZHANG Cuilan ZHAO Shujin |
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| Affiliation: | Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China |
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| Abstract: | |
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| Keywords: | Naja naja neuropathic pain glutamate-glial transporter 1 SNL |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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