人参皂苷Rg3对人肺鳞癌裸鼠移植瘤生长及VEGF bFGF表达的影响

目的:探讨人参皂苷Rg3对人肺鳞癌SK-MES-1细胞裸鼠移植瘤的生长及血管生成相关因子VEGF、bF-GF表达的影响。方法:通过皮下种植SK-MES-1细胞建立人肺鳞癌裸鼠移植瘤模型,成瘤后随机分为4组:对照组(生理盐水,0.5ml.d-1)、低剂量Rg3组(0.5mg·kg-1.d-1)、中剂量Rg3组(1mg·kg-1.d-1)和高剂量Rg3组(2mg·kg-1.d-1),每日灌胃1次,共28次。测量各组裸鼠移植瘤体积,光镜下观察移植瘤组织的形态;应用免疫组织化学和原位杂交法研究裸鼠移植瘤组织VEGF、bFGF的表达变化。结果:人参皂苷Rg3可显著抑制移植瘤的生长,各实验组抑瘤率分别达到41.47%、61.29%和80.14%,与对照组相比,肿瘤生长明显受抑,且具有剂量依赖性,各实验组移植瘤体积与对照组间比较P0.05,有统计学意义。Rg3能够抑制移植瘤VEGF、bFGFmRNA和蛋白表达,具有剂量依赖性,各实验组与对照组间比较均有统计学意义。结论:人参皂苷Rg3具有显著的抗肺鳞癌移植瘤作用,其机制可能与下调VEGF、bFGF表达有关。人参皂苷Rg3作为一种新的抗肺鳞癌药物,其作用机制有待于进一步研究。

Effects of Ginsenoside Rg3 on Growth and VEGF,bFGF Expressions of Transplanted Human Lung Squamous Cell Carcinoma in Nude Mice

Objective：To explore the inhibitory effects of Ginsenoside Rg3 on the growth and angiogenesis-related factors VEGF,bFGF expressions of human lung squamous cell carcinoma xenografts in nude mices.Methods：The xenografts derived from SK-MES-1 cells were established in BALB/C nude mice.Inoculated mice were randomly divided into control（normal saline,0.5mL·d-1）group,low dose Rg3（0.5mg·kg-1·d-1）group,moderate dose Rg3（1mg·kg-1·d-1）group and high dose Rg3（2mg·kg-1·d-1）group,daily oral administration,a total of 28 times.Tumor volume was measured.Tumor tissue was observed under microscope.The expression of vascular endothelial growth factor（VEGF）and basic fibroblast growth factor（bFGF）were detected by immunohistochemistry and in-situ hybridization.Results：Ginsenoside Rg3 could significantly suppress the growth of tumor and the inhibitory rate was 41.47%、61.29% and 80.14%,respectively.Rg3 caused a dose-dependent inhibitory effect on VEGF and bFGF protein and mRNA expression.Conclusion：Ginsenoside Rg3 could inhibit the growth of SK-MES-1 cell xenografts in nude mice.The down-regulation of VEGF and bFGF expression may play a key role in the antitumor effect of Rg3.Ginsenoside Rg3 should be examined further as a novel anti-neoplastic agent for the treatment of lung squamous cell carcinoma.