p16蛋白及其基因在原发性肝细胞癌中的表达缺失及意义

目的:通过p16蛋白及其基因在原发性肝细胞癌中的表达缺失研究,探讨p16基因与肝癌的关系.方法:收集我院原发性肝癌32例,用免疫组织化学SP法、聚合酶链式反应(PCR)方法分别检测肝癌及癌旁组织中p16蛋白的表达及其基因的缺失情况.结果:32例肝癌中,p16蛋白表达缺失率为2.5%(20/32),癌旁组织中p16蛋白表达缺失率为3.13%(1/32),两者差异有显著意义(P<0.01).P16蛋白的表达与肝癌分化程度关系密切(P<0.01).P16基因在癌组织中的缺失率25%(8/32,均为p16蛋白表达缺失者),在癌旁组织为3.13%(1/32),相差显著(P<0.05).结论:p16基因缺失与肝癌发生关系密切,提示p16蛋白可能是预测患者预后的一个指标,但仍需进一步确证.

A study on p16 protein expression and p16 gene deletion in primary hepatocellular carcinoma

AIM: To study the p16 protein expression and p16 gene deletion in hepatocellular carcinoma (HCC) and to evaluate the relationship between p16 gene and HCC. MEHTODS: The expression of p16 protein and the deletion of p16 gene were detected by immunohistochemistry and polymerase chain reaction (PCR) in 32 cases of HCC. RESULTS: The loss of p16 protein expression was observed in 20 cases of HCC (62.5%, 20/32) and in 1 case of tumor adjacent tissues (3.13%, 1/32), respectively ( P < 0.01). The loss of p16 protein expression was closely correlated with the poor histological differentiation of the tumor ( P < 0.01) . The deletion rate of p16 gene in HCC (25%,8/32) was significantly different from that in tumor adjacent hepatic tissues (3.13%, 1/32, P <0.05). CONCLUSION: There is a close relationship between hepatocarcinogenesis and p16 gene inactivation. The expression of p16 protein can be used as an index in predicting the prognosis.