~(99)Tc~m-EGFR-McAb及~(99)Tc~m-CD44-McAb单独及联合用于荷人肺腺癌裸鼠的显像研究

目的 评价99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合应用于荷人肺腺癌裸鼠的放射免疫显像效果.方法 建立荷人肺腺癌裸鼠动物模型;采用直接法99Tcm标记抗EGFR和CD44的单克隆抗体,运用柱层析法对标记抗体分离、纯化,并对标记、纯化后的抗体特性进行鉴定;利用99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合进行倚人肺腺癌裸鼠显像及体内分布研究.结果 99Tcm对EGFR-McAb和CD44-McAb的标记率分别为(91.5±3.8)%、(92.3±4.1)%,标记抗体比活度分别为(2.8±0.3)MBq/μl、(2.9±0.5)MBq/μl,放射化学纯度分别为(96.5±2.8)%、(96.2±3.1)%.放射免疫显像结果显示,肿瘤组织对2种标记抗体均有较高的摄取,2种标记抗体联合使用肿瘤部位的放射性浓聚明显高于2种标记抗体单独使用,通过ROI技术测得T/NT值分别为(5.58±0.46)、(2.72±0.22)、(2.30±0.18).标记抗体的体内分布规律与显像结果一致.结论 99Tcm-EGFR-McAb、99Tcm-CD44-McAb用于荷人肺腺癌裸鼠的放射免疫显像能得到较理想的靶/非靶比值,2种标记抗体的联合应用,可在一定程度上提高靶/非靶比值,优于一种标记抗体的单独使用.

Effects of radioimmunoimaging with ~(99)Tc~m-EGFR-McAb or ~(99)Tc~m-CD44-McAb or combined application of both on nude mice bearing human lung adenocarcinoma

Objective To evaluate the effects of radioimmunoimaging with 99Tcm-EGFR-McAb or 99Tcm-CD44-McAb or combined application of both on nude mice bearing lung adenocarcinoma. Methods Animal model of nude mice bearing lung adenocarcinoma was established. The direct labeling method of 99Tcm was applied to labeling monoclonal antibodies for EGFR and CD44, and then the properties of the labeling antibodies purified by SephadexG50 Column were identified. The radio-immuno-image as well as body distribution concerning ...