补肾和脉方治疗动脉粥样硬化炎症的效果及机制探讨

目的 研究补肾和脉方（HMF）对载脂蛋白 E 基因敲除（ApoE-/-）小鼠动脉粥样硬化（AS）的治疗效果及可能机制。方法 将 24 只 ApoE-/-小鼠随机分为高脂组、 HMF 组和阿托伐他汀组， 每组 8 只。高脂组给予高脂饲料（0.25%胆固醇和 15%可可脂） 喂养， HMF 组给予高脂饲料+HMF1.37 g/(kg·d)]喂养， 阿托伐他汀组给予高脂饲料+阿托伐他汀5 mg/(kg·d)]喂养。8 周后， 戊巴比妥钠（0.8%， 0.05 mL/10 g）腹腔注射麻醉， 取小鼠胸主动脉切片， 行常规 HE 染色观察胸主动脉斑块大小； 行免疫组化染色检测巨噬细胞 CD68、 肿瘤坏死因子 （TNF） -α、 白细胞介素 （IL） -1β 和 IL-8 的表达水平。并采用日本全自动生化分析仪测量小鼠总胆固醇 （TC） 和总三酰甘油 （TG） 水平。结果 HMF 组胸主动脉斑块大小， 胸主动脉斑块内 CD68、 TNF-α、 IL-1β和 IL-8 的表达水平， TC、 TG 水平均明显低于高脂组， 高于阿托伐他汀组 （均 P＜0.05）。结论 HMF 具有抗动脉硬化作用， 可抑制 AS 进展， 其机制可能与降低血脂， 减轻斑块炎症细胞浸润， 减少炎症因子分泌有关。

Therapeutic effects of Bu-shen-he-mai-fang (HMF) on inflammation in atherosclerosis

Objective To observe the therapeutic effect and mechanism of Bu-shen-he-mai-fang (HMF) on experi⁃ mental atherosclerosis (AS) in apolipoprotein-E knockout（ApoE-/-）mice. Methods Twenty-four male ApoE-/- mice were randomly divided into three groups including high-fat group (0.25% cholesterol and 15% cocoa butter), HMF group (highfat diet + HMF decoction 1.37 g/(kg·d)] and atrovastatin group (high-fat diet + atrovastatin 5 mg/(kg·d)], 8 mice for each group. The serum level of lipid was evaluated by a kit. The extent of AS was evaluated by HE staining. The macrophage infiltration and expression of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8) were evaluated by immunohistochemical staining. Results The serum level of lipid was lower in HMF group and atrovastatin group than that in high-fat group (P＜0.05). The degree of AS was significantly lower in HMF group and atrovastatin group than that in high-fat group. The macrophage infiltration, TNF-α, IL-1β and IL-8 expressions were significantly lower in HMF group and atrovastatin group than those in high-fat group (P＜0.05). Conclusion The results suggest that HMF significantly inhibits early atherosclerotic lesions by lowering serum lipid level and inhibiting inflammatory response.