| 神经肽Y对大鼠心肌细胞肌浆网钙的影响 |
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| 引用本文： | 张弋,苏涛,刘世明,董颀,陈敏生. 神经肽Y对大鼠心肌细胞肌浆网钙的影响[J]. 现代医院, 2009, 9(11): 9-11. DOI: 10.3969/j.issn.1671-332X.2009.11.004 |
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| 作者姓名： | 张弋 苏涛 刘世明 董颀 陈敏生 |
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| 作者单位： | 1. 广州医学院第二附属医院,广东广州,510260
2. 广州医学院生理教研室,广东广州,510182 |
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| 基金项目： | 国家自然科学基金资助项目，广东省自然科学基金资助项目 |
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| 摘 要： | 目的观察神经肽Y（NPY）刺激对大鼠心肌细胞肌浆网（Sarcoplasmic Reticulum,SR）内钙含量的影响。方法用100nmol·L^-1NPY刺激Sprague-Dawley乳鼠心肌细胞24h,用荧光染料Fluo-4AM负载胞浆钙,并用咖啡因诱导的胞浆钙瞬变（Caffeine-induced Ca2＋ transient,CCT）幅度来反映肌浆网内总钙负荷;用荧光染料Fluo-5NAM直接标记心肌细胞肌浆网内游离钙离子。所有钙影像均由LeicaSP2激光共聚焦显微镜记录。结果经100nmol·L^-1NPY刺激24h后,心肌细胞肌浆网内游离钙含量明显低于对照组（p〈0.01）;咖啡因诱导下钙瞬变幅度也低于对照组。结论长时间的NPY刺激可导致肌浆网内钙含量减少。
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| 关 键 词： | 神经肽Y 心肌细胞 肌浆网 钙瞬变 |
EFFECT OF NEUROPEPTIDE Y ON CALCIUM IN SARCOPLASMIC RETICULUM OF RAT CARDIOMYOCYTES. |
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| Affiliation: | ZHANG Yi, SU Tao, LIU Shiming, et al( Department of Emergency, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou City, Guangdong Province 510182 PRC) |
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| Abstract: | Objective To investigate the effect of neuropeptide Y （NPY） on calcium content in sarcoplasmic reticulum （SR） of rat cardiomyocytes.Methods Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY （100 nmol/L） for 24 h. Fluorescent assay with Fluo-4AM were used to detect Ca2＋ transients of cardiomyocytes and Fluo-5N AM were used to detect[Ca^2＋] in SR through laser scanning confocal microscope. The SR Ca^2＋ load was estimated by Caffeine-induced Ca^2＋ transient（CCT）.Results 24 hours after incubation, compared with control group, the concentration of free Ca^2 ＋ in SR（ [ Ca^2 ＋ ] ISR）was significantly decreased （p 〈 0. 05 ）, and the peak of CCT was attenuated.. Conclusions NPY lead to reduce the calcium content in sareoplasmic reticulum of rat eardiomyoeytes after a long period of stimulation. |
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| Keywords: | Neuropeptide Y Cardiomyocyte Sarcoplasmic reticulum Calcium transient |
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