| 鳖甲煎丸对人肾小球系膜细胞干预作用的研究 |
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| 引用本文： | 范焕芳,韩琳,王月华,陈志强,张雪娟.鳖甲煎丸对人肾小球系膜细胞干预作用的研究[J].辽宁中医杂志,2008(12). |
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| 作者姓名： | 范焕芳 韩琳 王月华 陈志强 张雪娟 |
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| 作者单位： | 河北医科大学中医院;北京中医药大学; |
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| 摘 要： | 目的:探讨中药鳖甲煎丸药物血清对人肾小球系膜细胞(MCs)增殖和凋亡作用的影响。方法:应用细胞培养技术,进行人MCs培养,采取血清药理学方法,制备鳖甲煎丸药物血清,利用脂多糖(LPS)为刺激因子,将培养的MCs分为空白对照组(简称空白组);鳖甲煎组;脂多糖组;脂多糖鳖甲煎组(简称脂甲组)。应用四甲基偶氮唑蓝(MTT)法和流式细胞仪检测MCs增殖、凋亡和细胞周期。结果:LPS刺激后,增殖指数明显提高,与空白组比较有明显差异(P<0.01);鳖甲煎丸药物血清能明显抑制人MCs增殖(P<0.01),并且使多数MCs阻滞在G0/G1期,能诱导人MCs发生凋亡。结论:抑制MCs增殖和促进凋亡可能是鳖甲煎丸延缓慢性肾脏疾病进展的部分作用机制。
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| 关 键 词： | 鳖甲煎丸 MCs 细胞增殖 细胞凋亡 细胞周期 |
Effect of Biejia Jian Pile on Proliferation,Apoptosis and Cell Cycle of Human Mesangial Cell |
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| FAN Huan-fang,HAN Lin,WANG Yue-hua,CHEN Zhi-qiang,ZHANG Xue-juan.Effect of Biejia Jian Pile on Proliferation,Apoptosis and Cell Cycle of Human Mesangial Cell[J].Liaoning Journal of Traditional Chinese Medicine,2008(12). |
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| Authors: | FAN Huan-fang HAN Lin WANG Yue-hua CHEN Zhi-qiang ZHANG Xue-juan |
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| Institution: | FAN Huan-fang1,HAN Lin2,WANG Yue-hua1,CHEN Zhi-qiang1,ZHANG Xue-juan1 (1.TCM Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China,2.Beijing University of Chinese Medicine,Beijing 100029,China) |
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| Abstract: | Objective:To study the role of Biejia Jian Pill(BJJP) on human mesangial cells in culture.Method:We adopt cell culture technique and blood serum pharmacology method in experiment.Mesangial cells were stimulated by lipopolysaccharide(LPS).There are four groups: control group;BJJP group;LPS group and LPS BJJP group.The MCs proliferation,apoptosis,and cell cycle of HMCs were measured by MTT assay and flow cytometry respectively.Result:After mesangial cells were stimulated by LPS,proliferation of mesangial cell... |
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| Keywords: | Biejia Jian Pill Human mesangial cell Proliferation Apoptosis Cell cycle |
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