Degradation,Residual Determination,and Cholinesterase Activity of Triclorfon in Piaractus mesopotamicus Holmberg (PACU) 1887 |
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| Authors: | Maria Isabel Mataqueiro Laura Satiko Okada Nakaghi Patrícia Kii Yamada Guilherme de Camargo Ferraz Antonio de Queiroz-Neto Georgino Honorato DeOliveira |
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| Affiliation: | 1. Faculdade de Ciências Agrárias e Veterinárias, UNESP, Univ Estadual Paulista Campus de Jaboticabal , Departamento de Morfologia e Fisiologia Animal, Laboratório de Famacologia e Fisiologia do Exercício Equino , Jaboticabal , S?o Paulo , Brazil mbel@fcav.unesp.br;3. Faculdade de Ciências Agrárias e Veterinárias, UNESP, Univ Estadual Paulista Campus de Jaboticabal , Departamento de Morfologia e Fisiologia Animal, Laboratório de Famacologia e Fisiologia do Exercício Equino , Jaboticabal , S?o Paulo , Brazil;4. Universidade Estadual Paulista (UNESP). Campus de Araraquara. Faculdade de Ciências Farmacêuticas, Departamento de princípios ativos Naturais e Toxicologia , Araraquara , S?o Paulo , Brazil |
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| Abstract: | The aim of this study was to determine the no-observable-adverse-effect concentration (NOAEC) for trichlorfon, an antiparasitic agent used in aquaculture, in Piractus mesopotamicus (pacu) using acetylcholinesterase (AChE) activity as an end point. Fish were exposed 24 h/d for 15 d to different concentrations of trichlorfon in tanks of water for which a curve of dissipation was previously determined. Analysis of trichlorfon in water and fish plasma using gas chromatography with electron capture detection (GC-ECD) enabled measurement of limit of detection (LOD) and limit of quantification (LOQ), respectively, to be 3 and 10 ppb. Thirty-six hours after trichlorfon dilution in water, the concentration was below the LOD, and data showed that plasma concentrations did not exceed the LOQ. Apart from the 6.25 μg/L, all concentrations of trichlorfon significantly inhibited plasma and brain AChE activity compared to controls. The AChE activity levels returned to control values in 7 d. These data may be useful to determine the concentration of trichlorfon that destroys parasites without producing adverse effects in fish. |
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