藻酸双酯钠和甘糖酯对急性心肌梗死患者红细胞变形能力保护作用的机制探讨

为探讨藻藻酯双酯钠(PSS)和甘糖酯(PGMS)对急性心肌梗死(AMI)患者红细胞变形能力(ED)保护作用的机制,检测了52例AMI患者红细胞滤过指数(EFI)、红细胞膜Na~ ,K~ -ATP酶和谷胱甘肽过氧化物酶GSH-Px活性及红细胞膜脂质过氧化物(LPO)的变化,同时观察了PSS和PGMS在体外对ED、Na~ ,K~ -ATP酶、GSH-Px和LPO的影响。结果显示,AMI患者EFI、LPO明显增高,Na~ ,K~ -ATP酶和GSH-Px活性明显降低,与对照组比较差异有极显著性(P<0.001)。AMI患者红细胞与PSS或PGMS在体外温育24h后,EFI、LPO明显降低,Na~ ,K~ -ATP酶和GSH-Px活性明显增高。与温育前比较差异有极显著性(P<0.001),与对照组比较差异无显著性(P>0.05)。提示PSS和PGMS具有保护和提高AMI患者ED的作用,其机制与增强红细胞膜ATP酶和抗氧化酶活性有关。

THE STUDY ON THE MECHANISM OF PROTECTING ERY- THROCYTE DEFORMABILITY OF PSS AND PGMS IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION

To explore the mechanism of protecting erythrocyte deformability (ED) of polysaccharide sulfate(PSS)and propyllene glycolmannurate sulfate(PGMS) in patients with acute myocardial infarction (AMI). The erythrocyte filtration index(EFI),erythrocgte membrane Na ,K -ATPase .glutathione peroxidase(GSH-Px)activity and lipid peroxide(LPO) were measured in 52 patients with AMI. Meanwhile the effects of PSS and PGMS on EFI, Na ,K -ATPase ,GSH-Px and LPO in AMI patients were observed in vitro. The results showed that the EFI and LPO were markedly higher ,Na ,K -ATP ase and GSH-Px were si-hnificantly lower in patients than those in control(P<0. 001),when AMI patients erythrocyte incubated with PSS or PGMS for 24 hours ,the EFI and LPO were markedly lower, Na ,K -ATPase and GSH-Px were markedly higher,as compared with the group before the incubation(P<0. 001),but there was no significance compared with controls before and after incubation (P>0. 05). These results indicate that PSS and PGMS could improve the ED in patients ,and the efficacy of PSS or PGMS was related to enchancement of erythrocyte membrane ATP-ase anci GSH-Px activity.