Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities |
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Authors: | Göksel Şener Halil Aksoy Özer Şehirli Meral Yüksel Cenk Aral Nursal Gedik Şule Çetinel Berrak Ç Yeğen |
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Institution: | (1) Department of Pharmacology, School of Pharmacy, Marmara University, Tıbbiye Cad. 34668, İstanbul, Turkey;(2) Department of Biochemistry, School of Pharmacy, Marmara University, Tıbbiye Cad. 34668, Istanbul, Turkey;(3) Vocational School of Health Related Professions, Marmara University, Tıbbiye Cad. 34668, Istanbul, Turkey;(4) Department of Medical Biology, School of Medicine, Marmara University, Tıbbiye Cad. 34668, Istanbul, Turkey;(5) Division of Biochemistry, Kasimpasa Military Hospital, Istanbul, Turkey;(6) Department of Histology-Embryology, School of Medicine, Marmara University, Tıbbiye Cad. 34668, Istanbul, Turkey;(7) Department of Physiology, School of Medicine, Marmara University, Tıbbiye Cad. 34668, Istanbul, Turkey |
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Abstract: | After intracolonic administration of trinitrobenzene sulphonic acid (TNBS), Sprague-Dawley rats were treated orally either
with saline or erdosteine (100 mg/kg per day), a sulfhydryl-containing antioxidant, for 3 days. On the 4th day, rats were
decapitated and distal colon was removed for the macroscopic and microscopic damage scoring, for the measurement of malondialdehyde
(MDA), glutathione (GSH) and collagen levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminescences (CL)
and DNA fragmentation. Lactate dehydrogenase (LDH) activity, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and antioxidant
capacity were assayed in blood samples. Colitis caused significant increases in the colonic CL values, macroscopic and microscopic
damage scores, MDA and collagen levels, MPO activity and DNA fragmentation, along with a significant decrease in tissue GSH
level. Similarly, serum cytokines and LDH were elevated in the saline-treated colitis group as compared with the control group.
On the other hand, erdosteine treatment reversed all these biochemical indices, and histopathologic alterations induced by
TNBS, suggesting that erdosteine protects the colonic tissue via its radical scavenging and antioxidant activities. |
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Keywords: | Erdosteine Colitis Oxidative damage Inflammation TNBS |
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