丙型肝炎病毒感染患者PBMC细胞因子的分泌水平

目的 :检测丙型肝炎病毒 (HCV)感染患者外周血单个核细胞(PBMC)体外培养后 ,培养上清中细胞因子分泌的水平 ,以反映HCV患者体内的免疫状况。方法 :患者PBMC培养上清中的细胞因子 (IL 2、IL 4、IL 10、IL 12、TNF γ、TNF α)采用ELISA进行检测。结果 :(1)HCV感染患者的PBMC培养 72h后 ,细胞因子检测结果表明 :与正常对照组相比较 ,HCV患者PBMC培养上清中TNF γ、IL 10和TNF α的水平明显升高 ,而没有检测到IL 2、IL 4、和IL 12的分泌产物。 (2 )轻、中度慢性肝炎 ,以及代偿期、失代偿期肝硬化患者间细胞因子的水平未见明显差异。结论 :(1)HCV感染患者体内细胞因子的分泌倾向于Th2型细胞因子占优势。 (2 )HCV感染患者PBMC分泌IL 2水平的降低可能是HCV免疫逃逸的原因之一

The cytokine secretion of peripheral blood mononucleocytes from patients infected with HCV

AIM: To detect the levels of cytokines secreted by PBMC from patients infected with HCV after culture for 72 hours in-vitro, so as to reflect the immune state of the HCV-infected patients. METHODS: The levels of cytokines in the culture supernatant of PBMCs were detected by ELISA. RESULTS: (1)Compared with the cytokine level of normal control, the levels of IFN-gamma, TNF-alpha, and IL-10 of HCV patients notably increased while IL-2, IL-4, and IL-12 were not detected in the culture supernatant of PBMCs from both normal control and HCV patients after culture for 72 hours. (2)There was no statistic difference in secretion of cytokines among the patients with mild, and moderate chronic hepatitis, and compensated or uncompensated cirrhosis. CONCLUSION: (1)PMBCs from the HCV-infected patients tend to secret Th2 type cytokines. (2)Lack of the IL-2 secretion from PBMCs of HCV patients may account for the immune escape of HCV.