新辅助化疗对乳腺癌细胞凋亡和增殖的影响

背景与目的：在实验研究中,已证实多种化疗药物都可引起乳腺癌细胞的凋亡。但在人体内进行有关化疗药物引起乳腺癌细胞凋亡的研究,还少有报道。本文研究新辅助化疗能否引起乳腺癌肿瘤细胞的凋亡以及对乳腺癌肿瘤细胞增殖的影响。方法：应用末端转移酶介导的dUTP切口末端标记法（TdT-mediated dUTP nick end labelling,TUNEL)及免疫组化的标记链菌亲和素生物素法（Labelled Streptavidin Biotin,LSAB),分别检测100例乳腺癌组织中肿瘤细胞的凋亡指数（Apoptotic index,AI)和乳腺癌肿瘤组织的增殖细胞核抗原（Proliferating cell nuclear antigen,PCNA)表达。结果：新辅助化疗组肿瘤细胞凋亡指数（AI）均数为7．47％,与对照组肿瘤细胞凋亡指数（AI）均数4．83％相比明显增高（P＜0．01）。新辅助化疗组肿瘤细胞增殖细胞核抗原（proliferation cell nuclear antigen,PCNA)阳性表达率均数为33．71％,与对照组PCNA阳性率均数51．52％相比明显降低（P＜0．01）。在新辅助化疗组及对照组两组病例中,肿瘤细胞凋亡指数（AI）与增殖细胞核抗原（PCNA）的阳性表达均呈负相关。结论：在人体乳腺癌组织中,新辅助化疗能诱导肿瘤细胞发生凋亡,并能抑制其增殖。

Affection of neoadjuvant chemotherapy on cell apoptosis and proliferation in breast cancer

Background &Objective:Although the researches had verifie d various kinds of chemotherapic age nts can cause the apoptosis of breast can cer cells,but the reports with in viv o data are still scarce.This paper is to investigate whether neoadjuvant chemotherapy c ould induce tumor cell apoptosis in b reast cancer patients,and its effec t on cell proliferation.Methods :Apoptosis index(AI )of tumor cells is assayed by TdT-medi ated dUTP nick end labeling(TUNEL)and proliferating cell nuclear antigen(PCNA)is examined by immunohistochemical labelled streptavidin biotin(LSAB)in100beast cancer samples.Results:Tumor cell AI in neoadjuvant group(x=7.47%)was significantly higher than that i n control group(x=4.83%)(P<0.01).PCNA positive expression rate in neoadjuvant group(x=33.71%)was significantly lower than that in con trol group(x=51.52%)(P<0.01).There was significant negative ass ociation between AI and PCNA in both neoadjuvant chemotherapy group and control group(P<0.05).Conclusion:Neoadjuvant chemotherapy could induce tumor cell apoptosis and inhibit tumor cell pr oliferation in human breast cancer.