氧化电位水对HBsAg破坏效果的试验及临床观察

目的 观察氧化电位水（EOW）在胃镜消毒中对乙型肝炎表面抗原（HBsAg）抗原性的破坏效果。方法 模拟现场试验用EOW与人工污染的胃液作用1min，观察其对HBsAg的破坏作用，以PBS作为对照组。现场试验以HBsAg阳性病人用后的污染胃镜，作为消毒前对照，经去有机物处理及EOW浸泡1min，胃镜表面涂抹采样，用ELISA检测HBsAg。结果 人工污染胃液（HBsAg平均S/N值为42.16)加入胃液量100倍的EOW作用1min后，HBsAg检测为阴性，对照组仍为阳性。临床污染胃镜（HBsAg平均S/N值为5.99）经清洁、消毒后HBsAg检测为阴性。结论 在消除有机物后，氧化电位水可有效的破坏污染胃镜HBsAg的抗原体。

Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water

OBJECTIVE: Observation on the effect of disinfection on gastroscope, contaminated by hepatitis B surface antigen (HBsAg) in the electrolyzed oxidizing water (EOW). METHODS: Contaminated gastric juice and serum was added to EOW for 1 minute. Positive control samples were treated with PBS instead of EOW in the same way. Gastroscopes used for hepatitis patients were immersed in the EOW for 1 minute after cleaning. Samples were collected before and after treatment. ELISA was used to test HBsAg. RESULTS: With mixed samples (average S/N = 42.16) of EOW, HBsAg became negative when diluted in 100 times. However, the HBsAg of positive control samples remained positive. After cleaning the gastroscope (average S/N = 5.99) immersed in EOW, HBsAg became negative. CONCLUSION: EOW was effective in destroying HBsAg which could be used for gastroscope disinfection.